Weber P C, Challberg M D, Nelson N J, Levine M, Glorioso J C
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109.
Cell. 1988 Jul 29;54(3):369-81. doi: 10.1016/0092-8674(88)90200-0.
The bacterial transposable element Tn5 was observed to undergo high-frequency sequence inversion when integrated into the herpes simplex virus type 1 (HSV-1) genome. Deletion analysis of the IS50 elements through which this recombination event occurred demonstrated the absence of cis-acting signals involved in the inversion process. Several observations suggested an intimate association of the recombination mechanism with HSV-1 DNA replication, including the ability of the seven viral genes that are essential for HSV-1 DNA synthesis to mediate Tn5 inversion in the absence of any other viral functions. Comparable results were obtained by using duplicate copies of the L-S junction of the HSV-1 genome. Thus inversion of the L and S components of the HSV-1 genome during productive infection does not appear to be a site-specific process, but rather is the result of generalized recombination mediated by the complex of gene products that replicate the viral DNA.
当细菌转座元件Tn5整合到单纯疱疹病毒1型（HSV-1）基因组中时，观察到它会发生高频序列倒位。对发生这种重组事件的IS50元件进行缺失分析表明，不存在参与倒位过程的顺式作用信号。多项观察结果表明，重组机制与HSV-1 DNA复制密切相关，包括HSV-1 DNA合成所必需的七个病毒基因在没有任何其他病毒功能的情况下介导Tn5倒位的能力。使用HSV-1基因组L-S连接处的重复拷贝也得到了类似的结果。因此，在生产性感染期间HSV-1基因组的L和S组分的倒位似乎不是一个位点特异性过程，而是由复制病毒DNA的基因产物复合物介导的普遍重组的结果。
