Clarke M F, Westin E, Schmidt D, Josephs S F, Ratner L, Wong-Staal F, Gallo R C, Reitz M S
Nature. 1984;308(5958):464-7. doi: 10.1038/308464a0.
The mechanism of leukaemogenic transformation by human T-cell leukaemia/lymphoma virus (HTLV), a retrovirus implicated in the aetiology of certain adult T-cell leukaemias and lymphomas, is unknown but is conceivably associated with the expression of the cellular analogues of retroviral oncogenes. The HUT-102 cell line, derived from a cutaneous T-cell lymphoma and infected with HTLV, expresses several cellular oncogenes. It is unusual among haemopoietic cell lines in that one of these is c-sis, the gene from which the oncogene v-sis of the simian sarcoma virus was derived, and perhaps the gene for platelet-derived growth factor (PDGF). To explore the possible role of c-sis expression in HTLV-induced disease, we have obtained cDNA clones of c-sis from HUT-102 cells. Here we describe two such clones and report that one of them transforms NIH-3T3 cells. This is the first example of transformation of NIH-3T3 cells by a human onc gene other than c-ras or Blym, as well as the first demonstration of transformation by a human cDNA clone.
人类T细胞白血病/淋巴瘤病毒（HTLV）是一种逆转录病毒，与某些成人T细胞白血病和淋巴瘤的病因有关，其致白血病转化机制尚不清楚，但可以想象与逆转录病毒癌基因的细胞类似物的表达有关。源自皮肤T细胞淋巴瘤并感染HTLV的HUT-102细胞系表达多种细胞癌基因。它在造血细胞系中很不寻常，因为其中之一是c-sis，猿猴肉瘤病毒的癌基因v-sis就是从这个基因衍生而来的，它可能也是血小板衍生生长因子（PDGF）的基因。为了探索c-sis表达在HTLV诱导疾病中的可能作用，我们从HUT-102细胞中获得了c-sis的cDNA克隆。在此我们描述两个这样的克隆，并报告其中一个克隆可转化NIH-3T3细胞。这是除c-ras或Blym之外，人类癌基因对NIH-3T3细胞进行转化的首个例子，也是人类cDNA克隆实现转化的首次证明。
