Lenz J, Celander D, Crowther R L, Patarca R, Perkins D W, Haseltine W A
Nature. 1984;308(5958):467-70. doi: 10.1038/308467a0.
Although the murine retrovirus SL3-3 is highly leukaemogenic, in both the structure of its genome and in its properties of replication in tissue culture it closely resembles the nonleukaemogenic retrovirus Akv (refs 3, 4). An earlier investigation of the properties of recombinant SL3-3-Akv viruses localized the major determinant of leukaemogenicity outside the env gene, in a region of the viral genome that includes the gag gene and the noncoding long terminal repeat (LTR). To localize the determinant of SL3-3's leukaemogenicity more precisely we have now construced a recombinant provirus containing the LTR of SL3-3 and the coding region of Akv. The leukaemogenicity of these recombinants demonstrates that the determinant of leukaemogenicity lies within the SL3-3 LTR. Nucleotide sequencing of the LTRs of SL3-3 and Akv shows that they differ by a set of changes in the region thought to contain a transcriptional enhancer element. We suggest that enhancer region sequences are the major determinants of leukaemogenicity in these viruses.
尽管鼠逆转录病毒SL3-3具有高度致白血病性，但在其基因组结构和组织培养中的复制特性方面，它与非致白血病性逆转录病毒Akv极为相似（参考文献3、4）。早期对重组SL3-3-Akv病毒特性的研究将致白血病性的主要决定因素定位在env基因之外，位于病毒基因组中包含gag基因和非编码长末端重复序列（LTR）的区域。为了更精确地定位SL3-3致白血病性的决定因素，我们现在构建了一种重组前病毒，它包含SL3-3的LTR和Akv的编码区。这些重组体的致白血病性表明致白血病性的决定因素位于SL3-3的LTR内。对SL3-3和Akv的LTR进行核苷酸测序表明，它们在被认为包含转录增强子元件的区域存在一系列差异。我们认为增强子区域序列是这些病毒致白血病性的主要决定因素。
