Thomas C Y, Nuckols J D, Murphy C, Innes D
Department of Internal Medicine, Microbiology, University of Virginia Health Sciences Center, Charlottesville 22908.
Virology. 1993 Apr;193(2):1013-7. doi: 10.1006/viro.1993.1218.
A 428-base pair region of the gag gene of a molecular clone of the SL3-3 murine leukemia virus (MuLV) was replaced with allelic sequences that contain the NB-tropic determinants of the Moloney MuLV. SL3-3NB, the ecotropic virus derived from this modified clone, rapidly induced T-cell lymphoblastic lymphomas in the Fv-1n mouse strains CWD and NIH Swiss and the Fv-1b strain, B10.Br. By Southern blot assay, each of the CWD tumors and all but one of the B10.Br tumors that were tested contained recombinant proviruses. The envelope genes of the B10.Br recombinant viruses retained the SL3-3NB 5' p15E (TM) gene sequences (type I env), whereas the CWD recombinants did not (type II env). The production of type I env recombinants by B10.Br mice is a characteristic that is shared with other mouse strains that express the H-2k haplotype. The results indicate that the inserted Moloney virus gag sequences conferred NB-tropism to SL3-3NB and did not interfere with the expression of SL3-3 pathogenic determinants or the formation of recombinant viruses.
将SL3 - 3小鼠白血病病毒（MuLV）分子克隆的gag基因的一个428个碱基对的区域替换为含有莫洛尼MuLV嗜NB细胞决定簇的等位基因序列。由此修饰克隆衍生而来的亲嗜性病毒SL3 - 3NB，在Fv - 1n小鼠品系CWD和NIH瑞士小鼠以及Fv - 1b品系B10.Br中迅速诱发T细胞淋巴母细胞淋巴瘤。通过Southern印迹分析，所检测的每个CWD肿瘤以及除一个之外的所有B10.Br肿瘤均含有重组前病毒。B10.Br重组病毒的包膜基因保留了SL3 - 3NB 5' p15E（TM）基因序列（I型env），而CWD重组体则没有（II型env）。B10.Br小鼠产生I型env重组体是与表达H - 2k单倍型的其他小鼠品系共有的特征。结果表明，插入的莫洛尼病毒gag序列赋予SL3 - 3NB嗜NB细胞性，并且不干扰SL3 - 3致病决定簇的表达或重组病毒的形成。
