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参与质粒分离和不相容性的Clo DF13质粒DNA区域的基因表达结构与调控

Structure and regulation of gene expression of a Clo DF13 plasmid DNA region involved in plasmid segregation and incompatibility.

作者信息

van den Elzen P J, Hakkaart M J, van Putten A J, Walters H H, Veltkamp E, Nijkamp H J

出版信息

Nucleic Acids Res. 1983 Dec 20;11(24):8791-808. doi: 10.1093/nar/11.24.8791.

DOI:10.1093/nar/11.24.8791
PMID:6324101
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326624/
Abstract

The bacteriocinogenic plasmid Clo DF13 contains genetic information involved in the accurate partitioning of the plasmid (parA and parB) as well as in incompatibility phenomena (incA, B, C and D). In this paper we report on the primary structure and regulation of gene expression of the 29% - 50% part of Clo DF13, containing the DNA regions incA, incB and parB as well as genes K and L. According to the results of our DNA sequence analysis, mapping of transposon insertions, RNA blotting and S1 mapping experiments, we conclude that: a) genes K and L are transcribed as one operon; transcription of this operon is initiated at a promoter (P2) located at 32.5% and proceeds in a clockwise direction. b) treatment of cells with mitomycin-C, significantly enhances transcription from P2, although this promoter is probably not directly repressed by lexA protein. c) Termination of transcription of this operon occurs between genes K and L, as well as distal to gene L. The possible role of gene products and/or sites, located within the 29-50% DNA region, in plasmid incompatibility and segregation is discussed.

摘要

产细菌素质粒Clo DF13含有与质粒精确分配（parA和parB）以及不相容现象（incA、B、C和D）相关的遗传信息。在本文中，我们报道了Clo DF13中29% - 50%部分的基因表达的一级结构和调控，该部分包含DNA区域incA、incB和parB以及基因K和L。根据我们的DNA序列分析、转座子插入图谱、RNA印迹和S1图谱实验结果，我们得出以下结论：a）基因K和L作为一个操纵子转录；该操纵子的转录起始于位于32.5%处的一个启动子（P2），并按顺时针方向进行。b）用丝裂霉素-C处理细胞可显著增强从P2的转录，尽管该启动子可能不直接受lexA蛋白抑制。c）该操纵子的转录终止发生在基因K和L之间以及基因L的远端。讨论了位于29 - 50% DNA区域内的基因产物和/或位点在质粒不相容性和分离中的可能作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f855/326624/cb82cbf80cf5/nar00369-0271-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f855/326624/41d058a959ac/nar00369-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f855/326624/ab02a3264770/nar00369-0270-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f855/326624/cb82cbf80cf5/nar00369-0271-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f855/326624/41d058a959ac/nar00369-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f855/326624/ab02a3264770/nar00369-0270-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f855/326624/cb82cbf80cf5/nar00369-0271-a.jpg

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