van den Elzen P J, Walters H H, Veltkamp E, Nijkamp H J
Nucleic Acids Res. 1983 Apr 25;11(8):2465-77. doi: 10.1093/nar/11.8.2465.
In this paper we present the complete nucleotide sequence of the bacteriocin gene of plasmid Clo DF13. According to the predicted aminoacid sequence the bacteriocin, cloacin DF13, consists of 561 aminoacids and has a molecular weight of 59,293 D. To obtain insight into the structure and function of specific parts of the cloacin molecule, we constructed a hydration profile and we predicted the secondary structure of the protein. According to our predictions, the N-terminus of cloacin DF13 (corresponding to the first 150-180 aminoacids) is relatively hydrophobic and is rich in glycine residues. The data obtained support previous findings that the N-terminal part of cloacin DF13 is involved in translocation of this protein across the cell membrane. The C-terminal part of the cloacin protein is rich in positively charged aminoacids; this might reflect the RNase activity located within this domain. A comparison of the bacteriocin genes and corresponding proteins of Clo DF13 and Col E1 did not reveal any homology at the level of either the nucleotide or the aminoacid sequence. The codon usage of both genes, however, exhibits striking similarities. The sequence data obtained during this study enabled us to present the nucleotide sequence of the entire cloacin operon. The structure of this operon and the regulation of expression of the genes, located within this operon, is discussed.
在本文中，我们展示了质粒Clo DF13的细菌素基因的完整核苷酸序列。根据预测的氨基酸序列，细菌素cloacin DF13由561个氨基酸组成，分子量为59,293道尔顿。为了深入了解cloacin分子特定部分的结构和功能，我们构建了水化分布图并预测了该蛋白质的二级结构。根据我们的预测，cloacin DF13的N端（对应于前150 - 180个氨基酸）相对疏水且富含甘氨酸残基。获得的数据支持了先前的发现，即cloacin DF13的N端部分参与该蛋白质跨细胞膜的转运。cloacin蛋白的C端富含带正电荷的氨基酸；这可能反映了该结构域内的核糖核酸酶活性。对Clo DF13和Col E1的细菌素基因及相应蛋白质的比较在核苷酸或氨基酸序列水平上未发现任何同源性。然而，这两个基因的密码子使用表现出显著的相似性。在本研究过程中获得的序列数据使我们能够展示整个cloacin操纵子的核苷酸序列。本文讨论了该操纵子的结构以及位于该操纵子内的基因的表达调控。
