Fornari C S, Watkins M, Kaplan S
Plasmid. 1984 Jan;11(1):39-47. doi: 10.1016/0147-619x(84)90005-2.
Ten strains of Rhodopseudomonas sphaeroides were analyzed by agarose gel electrophoresis for plasmid DNA content and, by filter-hybridizations, for their molecular relationships. All strains examined contained at least one plasmid. Several strains carried as many as six different plasmid species with sizes ranging from 42 to 140 kilobases (kb). Those larger than 89 kb showed extensive homology with each other; the 42-kb plasmid of R. sphaeroides strain 2.4.1 was homologous to the smaller plasmid DNA of three other strains. A partial map of the 42-kb plasmid derived from R. sphaeroides 2.4.1 was prepared by analysis of restriction endonuclease digests. Cross-hybridization among the large plasmids indicated that those present in any one strain of R. sphaeroides showed homology to one or more of the large plasmids detected in strains L and 2.4.1.
通过琼脂糖凝胶电泳分析了十株球形红假单胞菌的质粒DNA含量，并通过滤膜杂交分析了它们之间的分子关系。所有检测的菌株都至少含有一个质粒。几个菌株携带多达六种不同的质粒种类，大小从42到140千碱基（kb）不等。那些大于89 kb的质粒彼此之间显示出广泛的同源性；球形红假单胞菌2.4.1菌株的42-kb质粒与其他三个菌株的较小质粒DNA同源。通过分析限制性内切酶消化产物，制备了源自球形红假单胞菌2.4.1的42-kb质粒的部分图谱。大质粒之间的交叉杂交表明，球形红假单胞菌任何一个菌株中存在的大质粒与在L菌株和2.4.1菌株中检测到的一个或多个大质粒显示出同源性。
