Nereng K S, Kaplan S
Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center Medical School, Houston, Texas 77030, USA.
J Bacteriol. 1999 Mar;181(5):1684-8. doi: 10.1128/JB.181.5.1684-1688.1999.
Pulsed-field gel electrophoresis following the use of rare cutting restriction endonucleases together with Southern hybridization, using markers distributed on chromosomes I and II of Rhodobacter sphaeroides 2.4.1, has been used to examine approximately 25 strains of R. sphaeroides in an effort to assess the occurrence of genome complexity in these strains. The results suggest that genome complexity is widespread and is accompanied by substantial genomic heterogeneity.
使用稀有切割限制内切酶后进行脉冲场凝胶电泳，并结合Southern杂交，利用分布在球形红细菌2.4.1的染色体I和II上的标记，已对约25株球形红细菌进行检测，以评估这些菌株中基因组复杂性的发生情况。结果表明，基因组复杂性普遍存在，并伴随着大量的基因组异质性。
