Dickgiesser N
Plasmid. 1984 Jan;11(1):99-101. doi: 10.1016/0147-619x(84)90013-1.
Haemophilus influenzae plasmid pVe445 (4.6 MDa) has been compared with Neisseria gonorrhoeae plasmids pNG18 (3.3 MDa) and pNG10 (4.6 MDa) by heteroduplex and restriction enzyme analysis. This study demonstrates that pVe445 differs from pNG18 only that it carries an additional 1.3-MDa DNA fragment between coordinates 0.9 and 2.2. Heteroduplex studies demonstrated that pNG10 and pVe445 are indistinguishable whereas restriction endonuclease analysis indicated minor differences in the distribution of AluI sites.
通过异源双链分析和限制性内切酶分析，对流感嗜血杆菌质粒pVe445（4.6兆道尔顿）与淋病奈瑟菌质粒pNG18（3.3兆道尔顿）和pNG10（4.6兆道尔顿）进行了比较。本研究表明，pVe445与pNG18的不同之处仅在于它在坐标0.9和2.2之间携带一个额外的1.3兆道尔顿DNA片段。异源双链研究表明pNG10和pVe445难以区分，而限制性内切酶分析表明AluI位点分布存在微小差异。
