Elferink J G
Res Commun Chem Pathol Pharmacol. 1984 Feb;43(2):339-42.
Stimulation of human neutrophils with phorbol myristate acetate results in a metabolic burst, which can be measured as an enhanced cytochrome c reduction or NBT reduction. There is more NBT reduction than cytochrome c reduction. When cytochrome c and NBT are simultaneously present the reduction of each is about the same as when either cytochrome c or NBT is present. Whereas cytochrome c reduction is completely annihilated by externally added superoxide dismutase, NBT reduction is diminished to a lesser extent under the same conditions. It is concluded that cytochrome c reduction only measures extracellularly released superoxide, whereas NBT may be reduced by extracellular superoxide or other molecules as well; thus NBT measures another aspect of the metabolic burst.
用佛波醇肉豆蔻酸酯乙酸盐刺激人中性粒细胞会导致代谢爆发，这可以通过增强的细胞色素c还原或NBT还原进行测量。NBT还原比细胞色素c还原更多。当细胞色素c和NBT同时存在时，每种物质的还原程度与单独存在细胞色素c或NBT时大致相同。虽然细胞色素c还原会被外部添加的超氧化物歧化酶完全消除，但在相同条件下NBT还原的程度会有较小程度的降低。结论是细胞色素c还原仅测量细胞外释放的超氧化物，而NBT可能也会被细胞外超氧化物或其他分子还原；因此NBT测量了代谢爆发的另一个方面。
