Grant R A, Webster R E
Virology. 1984 Mar;133(2):329-40. doi: 10.1016/0042-6822(84)90399-4.
The region of the bacteriophage f1 genome near the gene IV/intergenic region (IG) junction previously was shown to contain a sequence necessary for efficient packaging of single-stranded (SS) DNA into phage particles (the f1 "morphogenetic signal") [G. P. Dotto, V. Enea, and N. D. Zinder (1981) Virology 114, 463-473]. The DNA content of f1 phage/pBR322 chimeric plasmid-transformed, phage-infected bacteria has been investigated. The chimeric plasmids, constructed by Dotto et al. (1981), contained the f1 origins of DNA replication and, in some cases, also contained the "morphogenetic signal." Chimeric plasmid SS DNA was detected in phage-infected bacteria harboring any one of these chimeric plasmids, and the majority of this SS DNA was found complexed to the phage gene V protein. Therefore, the "morphogenetic signal" is not required for the formation of the gene V protein/f1 SS DNA complex but instead must function at a later stage of filamentous phage morphogenesis.
噬菌体f1基因组中靠近基因IV/基因间区域（IG）交界处的区域，先前已被证明含有将单链（SS）DNA有效包装到噬菌体颗粒中所必需的序列（f1“形态发生信号”）[G.P.多托、V.埃内亚和N.D.津德（1981年），《病毒学》114卷，463 - 473页]。已对f1噬菌体/pBR322嵌合质粒转化的、受噬菌体感染的细菌的DNA含量进行了研究。多托等人（1981年）构建的嵌合质粒含有f1 DNA复制起点，在某些情况下，还含有“形态发生信号”。在携带这些嵌合质粒中任何一种的受噬菌体感染的细菌中检测到了嵌合质粒SS DNA，并且发现这种SS DNA的大部分与噬菌体基因V蛋白复合。因此，基因V蛋白/f1 SS DNA复合物的形成不需要“形态发生信号”，相反，它必定在丝状噬菌体形态发生的后期发挥作用。
