Hirschhorn R R, Mercer W E, Liu H T, Baserga R
Virology. 1984 Apr 15;134(1):220-9. doi: 10.1016/0042-6822(84)90287-3.
Thymidine kinase-deficient syrian hamster cells were cotransfected with recombinant plasmids containing the thymidine kinase (TK) gene of Herpes Simplex Virus Type 1, and either intact or partially deleted SV40 T antigen-coding genes. The transformants were selected by their ability to grow in gHAT medium. After selection and cloning, the TK-positive transformants that also expressed T antigen were tested for the extent of their transformation with respect to a number of characteristics, which included saturation density, ability to grow in soft agar, resistance to butyrate and to dibutyryl-cAMP, and plating efficiency. The combined results of these various tests indicate that cells containing partially deleted SV40 T antigen-coding genes are less transformed than cells containing an intact SV40 T antigen-coding gene. However, the amounts of T antigen are lower in cells transformed by deletion mutants than in cells transformed by wild-type T antigen-coding gene. Our data indicate that both the quantity and the quality of T antigen may be important in determining the degree of transformation in Syrian hamster cells.
将胸苷激酶缺陷型叙利亚仓鼠细胞与含有1型单纯疱疹病毒胸苷激酶（TK）基因以及完整或部分缺失的SV40 T抗原编码基因的重组质粒共转染。通过它们在gHAT培养基中生长的能力来选择转化体。经过选择和克隆后，对那些也表达T抗原的TK阳性转化体就若干特性的转化程度进行检测，这些特性包括饱和密度、在软琼脂中生长的能力、对丁酸盐和二丁酰环磷腺苷的抗性以及接种效率。这些各种测试的综合结果表明，含有部分缺失的SV40 T抗原编码基因的细胞比含有完整SV40 T抗原编码基因的细胞转化程度低。然而，缺失突变体转化的细胞中T抗原的量比野生型T抗原编码基因转化的细胞中的T抗原量要低。我们的数据表明，T抗原的数量和质量在决定叙利亚仓鼠细胞的转化程度方面可能都很重要。
