Tsaritsidze M A, Lomsadze B A, Shengeliia M G
Vopr Med Khim. 1984 Jan-Feb;30(1):17-20.
Formation of complexes between carcinogenic polycyclic hydrocarbons and lysosomal membranes, exhibiting high inhibitory activity, was studied. Separation of lysosomal membranes into protein and lipid moities and further study of their inhibitory activity enabled to demonstrate that the fraction of lysosomal lipids was responsible for the phenomenon. Elimination of membrane lipids decreased the binding of benz(alpha)pyrene with lysosomes. The carcinogen entering into lysosomal membrane appears to substitute cholesterol in the phospholipid complex, being introduced in the fatty acid chain of phospholipids, thus altering the conformation of protein and protein-lipid complexes in the lysosomal membrane with the subsequent liberation of the lysosomal hydrolytic enzymes into cytoplasm.
对具有高抑制活性的致癌多环烃与溶酶体膜之间复合物的形成进行了研究。将溶酶体膜分离为蛋白质和脂质部分，并进一步研究它们的抑制活性，结果表明溶酶体脂质部分是造成该现象的原因。去除膜脂会降低苯并(a)芘与溶酶体的结合。进入溶酶体膜的致癌物似乎取代了磷脂复合物中的胆固醇，被引入磷脂的脂肪酸链中，从而改变了溶酶体膜中蛋白质和蛋白质-脂质复合物的构象，随后溶酶体水解酶释放到细胞质中。
