Chelmicka-Schorr E, Sportiello M G, Atweh S F, Arnason B G
Ann Neurol. 1984 Jan;15(1):96-9. doi: 10.1002/ana.410150117.
Rat C-6 glioma serves as an experimental model for human glioma. C-6 glioma cells carried to high culture passages in medium containing 10% fetal bovine serum when injected in vivo are unresponsive to treatment with the beta-adrenergic agonist isoproterenol and the phosphodiesterase inhibitor papaverine. When C-6 glioma cells are kept in culture in serum-containing medium, beta-adrenergic receptor density falls and, concomitantly, ability to accumulate cyclic adenosine monophosphate in response to stimulation with catecholamines declines. Responsiveness to treatment in vivo with a beta-adrenergic agonist was restored when C-6 glioma cells were cultured in serum-free defined medium prior to systemic injection into rats. Culturing of C-6 glioma cells in serum-free medium significantly increases the number of beta-adrenergic receptors when compared with C-6 glioma cells grown in serum-containing medium.
大鼠C-6胶质瘤可作为人类胶质瘤的实验模型。当在含有10%胎牛血清的培养基中传代培养至高代数后，将C-6胶质瘤细胞注射到体内时，它们对β-肾上腺素能激动剂异丙肾上腺素和磷酸二酯酶抑制剂罂粟碱的治疗无反应。当C-6胶质瘤细胞在含血清的培养基中培养时，β-肾上腺素能受体密度下降，同时，对儿茶酚胺刺激产生反应而积累环磷酸腺苷的能力也下降。当C-6胶质瘤细胞在全身注射到大鼠体内之前，先在无血清限定培养基中培养时，其对β-肾上腺素能激动剂体内治疗的反应性得以恢复。与在含血清培养基中生长的C-6胶质瘤细胞相比，在无血清培养基中培养C-6胶质瘤细胞可显著增加β-肾上腺素能受体的数量。
