Stanley P, Vivona G, Atkinson P H
Arch Biochem Biophys. 1984 Apr;230(1):363-74. doi: 10.1016/0003-9861(84)90119-x.
Carbohydrate moieties derived from the G glycoprotein of Vesicular Stomatitis Virus (VSV) grown in parental Chinese hamster ovary (CHO) cells and the glycosylation mutant Lec4 have been analyzed by high-field 1H NMR spectroscopy. The major glycopeptides of CHO/VSV and Lec4/VSV were purified by their ability to bind to concanavalin A-Sepharose. The carbohydrates in this fraction are of the biantennary, complex type with heterogeneity in the presence of alpha(2,3)-linked sialic acid and alpha (1,6)-linked fucose residues. A minor CHO/VSV glycopeptide fraction, which does not bind to concanavalin A-Sepharose but which binds to pea lectin-agarose, was also investigated by 1H NMR spectroscopy. These carbohydrates are complex moieties which appear to contain N-acetylglucosamine in beta(1,6) linkage. Their spectral properties are most similar to those of a triantennary complex oligosaccharide containing a 2, 6-disubstituted mannose alpha (1,6) residue. Carbohydrates of this type are not found among the glycopeptides of VSV grown in the Lec4 CHO glycosylation mutant.
利用高场1H核磁共振光谱法，对在亲代中国仓鼠卵巢（CHO）细胞和糖基化突变体Lec4中培养的水疱性口炎病毒（VSV）G糖蛋白衍生的碳水化合物部分进行了分析。通过与伴刀豆球蛋白A-琼脂糖结合的能力，纯化了CHO/VSV和Lec4/VSV的主要糖肽。该部分中的碳水化合物为双天线复杂型，在存在α(2,3)-连接的唾液酸和α(1,6)-连接的岩藻糖残基方面具有异质性。还通过1H核磁共振光谱法研究了一个次要的CHO/VSV糖肽部分，该部分不与伴刀豆球蛋白A-琼脂糖结合，但与豌豆凝集素-琼脂糖结合。这些碳水化合物是复杂部分，似乎含有β(1,6)连接的N-乙酰葡糖胺。它们的光谱特性与含有2,6-二取代甘露糖α(1,6)残基的三天线复杂寡糖最为相似。在Lec4 CHO糖基化突变体中培养的VSV糖肽中未发现这种类型的碳水化合物。
