经清道夫途径的甲醛处理血清白蛋白在肝内皮细胞中的内吞作用。
Endocytosis of formaldehyde-treated serum albumin via scavenger pathway in liver endothelial cells.
作者信息
Blomhoff R, Eskild W, Berg T
出版信息
Biochem J. 1984 Feb 15;218(1):81-6. doi: 10.1042/bj2180081.
Abstract
Denatured or modified proteins (including albumin and low-density lipoprotein) are catabolized in vitro via scavenger receptors. We have studied the distribution of formaldehyde-denatured albumin in rat liver cells after intravenous injection of tracer doses of the protein. At 12 min after injection, most of the formaldehyde-denatured albumin (about 70% of the injected dose) was recovered in liver endothelial cells. Furthermore, isolated liver endothelial cells in suspension and in surface culture took up formaldehyde-denatured albumin by receptor-mediated endocytosis. Our data indicate that the scavenger receptor in liver is mainly located on the endothelial cells. Implications for the catabolism of low-density lipoproteins are discussed.
摘要
变性或修饰的蛋白质（包括白蛋白和低密度脂蛋白）在体外通过清道夫受体进行分解代谢。我们研究了静脉注射示踪剂量的蛋白质后，甲醛变性白蛋白在大鼠肝细胞中的分布情况。注射后12分钟，大部分甲醛变性白蛋白（约占注射剂量的70%）在肝内皮细胞中被发现。此外，悬浮培养和贴壁培养的分离肝内皮细胞通过受体介导的内吞作用摄取甲醛变性白蛋白。我们的数据表明，肝脏中的清道夫受体主要位于内皮细胞上。文中还讨论了其对低密度脂蛋白分解代谢的意义。
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