通过一种新型电子顺磁共振方法直接测量星蓝蛋白的自交换速率。
Direct measurement of the self-exchange rate of stellacyanin by a novel e.p.r. method.
作者信息
Dahlin S, Reinhammar B, Wilson M T
出版信息
Biochem J. 1984 Mar 1;218(2):609-14. doi: 10.1042/bj2180609.
Abstract
A method for reconstituting the blue copper protein stellacyanin with the stable copper isotopes 63Cu and 65Cu is reported. Small differences in the e.p.r. spectra of the two isotopic forms of stellacyanin have been used to monitor the electron self-exchange reaction of stellacyanin by rapid-freeze e.p.r. methods. The self-exchange rate constant (k11) for stellacyanin has been determined as 1.2 X 10(5) M-1 X S-1 at 20 degrees C. This value is in close agreement with values obtained from less-direct methods.
摘要
报道了一种用稳定铜同位素63Cu和65Cu重构蓝铜蛋白星蓝蛋白的方法。利用星蓝蛋白两种同位素形式的电子顺磁共振（e.p.r.）光谱的微小差异，通过快速冷冻e.p.r.方法监测星蓝蛋白的电子自交换反应。已确定星蓝蛋白在20℃时的自交换速率常数（k11）为1.2×10(5) M-1×S-1。该值与通过不太直接的方法获得的值非常一致。
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