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钙在促黄体生成激素释放激素激动剂（ICI 118630）刺激大鼠睾丸间质细胞类固醇生成中的作用。

The role of calcium in luteinizing hormone-releasing hormone agonist (ICI 118630)-stimulated steroidogenesis in rat Leydig cells.

作者信息

Sullivan M H, Cooke B A

出版信息

Biochem J. 1984 Mar 1;218(2):621-4. doi: 10.1042/bj2180621.

DOI:10.1042/bj2180621
PMID:6324760
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1153380/
Abstract

The luteinizing hormone-releasing hormone (LHRH) agonist ICI 118630 was found to increase testosterone production in purified rat testis Leydig cells in a concentration- and time-dependent manner, but no consistent changes in cyclic AMP levels were detectable. The stimulation of steroidogenesis by LHRH agonist was found to be dependent on the concentration of Ca2+ in the incubation medium; at least 1 mM was required. The calcium ionophore A23187 mimicked the effects of the LHRH agonist on steroidogenesis, and addition of both compounds together did not further increase testosterone production. The calcium ionophore caused a small increase in cyclic AMP which was independent of the concentration of the ionophore and of the calcium concentrations. The evidence obtained in this study indicates that LHRH agonist-stimulated steroidogenesis in rat testis Leydig cells is primarily mediated by calcium and not cyclic AMP.

摘要

促黄体生成激素释放激素（LHRH）激动剂ICI 118630被发现以浓度和时间依赖性方式增加纯化的大鼠睾丸间质细胞中的睾酮生成，但未检测到环磷酸腺苷（cAMP）水平有一致变化。发现LHRH激动剂对类固醇生成的刺激依赖于孵育培养基中Ca2+的浓度；至少需要1 mM。钙离子载体A23187模拟了LHRH激动剂对类固醇生成的作用，同时添加这两种化合物并不会进一步增加睾酮生成。钙离子载体导致cAMP有小幅增加，这与离子载体的浓度和钙浓度无关。本研究获得的证据表明，LHRH激动剂刺激大鼠睾丸间质细胞中的类固醇生成主要由钙介导，而非cAMP。

相似文献

1
The role of calcium in luteinizing hormone-releasing hormone agonist (ICI 118630)-stimulated steroidogenesis in rat Leydig cells.钙在促黄体生成激素释放激素激动剂（ICI 118630）刺激大鼠睾丸间质细胞类固醇生成中的作用。
Biochem J. 1984 Mar 1;218(2):621-4. doi: 10.1042/bj2180621.
2
The effect of calcium on the potentiation of LH-stimulated steroidogenesis and inhibition of LH-stimulated cyclic AMP production by LHRH agonist (ICI 118630) in rat Leydig cells.钙对大鼠睾丸间质细胞中促黄体生成素（LH）刺激的类固醇生成增强作用以及促黄体生成素释放激素（LHRH）激动剂（ICI 118630）对LH刺激的环磷酸腺苷（cAMP）生成的抑制作用的影响。
Mol Cell Endocrinol. 1984 Jan;34(1):17-22. doi: 10.1016/0303-7207(84)90154-0.
3
The role of Ca2+ in steroidogenesis in Leydig cells. Stimulation of intracellular free Ca2+ by lutropin (LH), luliberin (LHRH) agonist and cyclic AMP.钙离子在睾丸间质细胞类固醇生成中的作用。促黄体素（LH）、促性腺激素释放激素（LHRH）激动剂和环磷酸腺苷对细胞内游离钙离子的刺激作用。
Biochem J. 1986 May 15;236(1):45-51. doi: 10.1042/bj2360045.
4
LHRH agonist decreases LH- but not forskolin-stimulated cyclic AMP levels in rat Leydig cells in vitro.促性腺激素释放激素激动剂可降低体外培养的大鼠睾丸间质细胞中促黄体生成素刺激的环磷酸腺苷水平，但不影响福斯高林刺激的环磷酸腺苷水平。
Mol Cell Endocrinol. 1984 Jun;36(1-2):115-22. doi: 10.1016/0303-7207(84)90090-x.
5
Stimulation and inhibition by LHRH analogues of cultured rat Leydig cell function and lack of effect on mouse Leydig cells.促黄体生成素释放激素类似物对培养的大鼠睾丸间质细胞功能的刺激和抑制作用以及对小鼠睾丸间质细胞无影响。
Mol Cell Endocrinol. 1982 Jun;27(1):31-44. doi: 10.1016/0303-7207(82)90060-0.
6
Mechanism of action of gonadotropin-releasing hormone-stimulated Leydig cell steroidogenesis. I. The stimulatory effect is calcium dependent and not mediated by cyclic nucleotides.促性腺激素释放激素刺激的睾丸间质细胞类固醇生成的作用机制。I. 刺激作用依赖于钙，而非由环核苷酸介导。
J Androl. 1984 May-Jun;5(3):193-200. doi: 10.1002/j.1939-4640.1984.tb02392.x.
7
Modulation and role of Ca2+ in LH and LHRH agonist action in rat Leydig cells.钙离子（Ca2+）在大鼠睾丸间质细胞中对促黄体生成素（LH）和促性腺激素释放激素（LHRH）激动剂作用的调节及作用
Med Biol. 1986;63(5-6):259-63.
8
Stimulatory effect of LHRH and its agonists on Leydig cell steroidogenesis in vitro.
Mol Cell Endocrinol. 1982 Apr;26(1-2):141-50. doi: 10.1016/0303-7207(82)90012-0.
9
The effects of calcium ionophore A23187 on interstitial cell steroidogenesis.钙离子载体A23187对间质细胞类固醇生成的影响。
Biochim Biophys Acta. 1980 Jan 17;627(2):157-64. doi: 10.1016/0304-4165(80)90317-7.
10
Stimulation of cholesterol side-chain cleavage by a luteinizing-hormone-releasing hormone (luliberin) agonist (ICI 118630) in rat Leydig cells.促黄体生成激素释放激素（促性腺激素释放素）激动剂（ICI 118630）对大鼠睾丸间质细胞胆固醇侧链裂解的刺激作用。
Biochem J. 1983 Dec 15;216(3):747-52. doi: 10.1042/bj2160747.

引用本文的文献

1
Effects of calmodulin and lipoxygenase inhibitors on LH (lutropin)- and LHRH (luliberin)-agonist-stimulated steroidogenesis in rat Leydig cells.钙调蛋白和脂氧合酶抑制剂对大鼠睾丸间质细胞中促黄体生成素（促黄体素）和促性腺激素释放激素（促性腺素释放素）激动剂刺激的类固醇生成的影响。
Biochem J. 1985 Nov 15;232(1):55-9. doi: 10.1042/bj2320055.
2
The role of Ca2+ in steroidogenesis in Leydig cells. Stimulation of intracellular free Ca2+ by lutropin (LH), luliberin (LHRH) agonist and cyclic AMP.钙离子在睾丸间质细胞类固醇生成中的作用。促黄体素（LH）、促性腺激素释放激素（LHRH）激动剂和环磷酸腺苷对细胞内游离钙离子的刺激作用。
Biochem J. 1986 May 15;236(1):45-51. doi: 10.1042/bj2360045.

本文引用的文献

1
Stimulation of Leydig cell testosterone secretion in vitro and in vivo in hypophysectomized rats by an agonist of luteinizing hormone releasing hormone.促黄体生成素释放激素激动剂对去垂体大鼠体外和体内睾丸间质细胞睾酮分泌的刺激作用
Biochem Biophys Res Commun. 1982 Jun 30;106(4):1210-7. doi: 10.1016/0006-291x(82)91241-4.
2
Stimulation of progesterone and prostaglandin E accumulation by luteinizing hormone-releasing hormone (LHRH) and LHRH analogs in rat granulosa cells.促黄体生成激素释放激素（LHRH）及LHRH类似物对大鼠颗粒细胞孕酮和前列腺素E蓄积的刺激作用。
Endocrinology. 1982 Jan;110(1):146-52. doi: 10.1210/endo-110-1-146.
3
Luteinizing hormone releasing hormone agonists induce ovulation in hypophysectomized proestrous rats: direct ovarian effect.促黄体生成素释放激素激动剂可诱导垂体切除的动情前期大鼠排卵：直接的卵巢效应。
Life Sci. 1981 Jul 13;29(2):185-92. doi: 10.1016/0024-3205(81)90287-3.
4
Mechanism of action of gonadotropin-releasing hormone. Involvement of phospholipid turnover in luteinizing hormone release.促性腺激素释放激素的作用机制。磷脂周转在促黄体生成素释放中的作用。
J Biol Chem. 1981 Mar 10;256(5):2226-9.
5
Inhibition of gonadotropin-releasing hormone-stimulated luteinizing hormone release by pimozide: evidence for a site of action after calcium mobilization.匹莫齐特对促性腺激素释放激素刺激的促黄体生成素释放的抑制作用：钙动员后作用位点的证据。
Endocrinology. 1981 Oct;109(4):1122-6. doi: 10.1210/endo-109-4-1122.
6
Effect of LHRH on incorporation of [32P]-orthophosphate into phosphatidylinositol by dispersed anterior pituitary cells.促黄体生成素释放激素对分散的垂体前叶细胞将[32P] - 正磷酸盐掺入磷脂酰肌醇的影响。
Mol Cell Endocrinol. 1982 Sep;28(1):55-63. doi: 10.1016/0303-7207(82)90040-5.
7
The effect of cell damage on the density and steroidogenic capacity of rat testis Leydig cells, using an NADH exclusion test for determination of viability.利用NADH排除试验测定活力，研究细胞损伤对大鼠睾丸间质细胞密度和类固醇生成能力的影响。
J Steroid Biochem. 1983 Apr;18(4):411-4. doi: 10.1016/0022-4731(83)90059-6.
8
The effect of calcium on the potentiation of LH-stimulated steroidogenesis and inhibition of LH-stimulated cyclic AMP production by LHRH agonist (ICI 118630) in rat Leydig cells.钙对大鼠睾丸间质细胞中促黄体生成素（LH）刺激的类固醇生成增强作用以及促黄体生成素释放激素（LHRH）激动剂（ICI 118630）对LH刺激的环磷酸腺苷（cAMP）生成的抑制作用的影响。
Mol Cell Endocrinol. 1984 Jan;34(1):17-22. doi: 10.1016/0303-7207(84)90154-0.
9
The deleterious effect of mechanical dissociation of rat testes on the functional activity and purification of Leydig cells using Percoll gradients.大鼠睾丸机械分离对使用Percoll梯度法分离睾丸间质细胞功能活性及纯化的有害影响。
Int J Androl. 1982 Apr;5(2):191-5. doi: 10.1111/j.1365-2605.1982.tb00247.x.
10
Stimulation and inhibition by LHRH analogues of cultured rat Leydig cell function and lack of effect on mouse Leydig cells.促黄体生成素释放激素类似物对培养的大鼠睾丸间质细胞功能的刺激和抑制作用以及对小鼠睾丸间质细胞无影响。
Mol Cell Endocrinol. 1982 Jun;27(1):31-44. doi: 10.1016/0303-7207(82)90060-0.