钙在促黄体生成激素释放激素激动剂（ICI 118630）刺激大鼠睾丸间质细胞类固醇生成中的作用。
The role of calcium in luteinizing hormone-releasing hormone agonist (ICI 118630)-stimulated steroidogenesis in rat Leydig cells.
作者信息
Sullivan M H, Cooke B A
出版信息
Biochem J. 1984 Mar 1;218(2):621-4. doi: 10.1042/bj2180621.
Abstract
The luteinizing hormone-releasing hormone (LHRH) agonist ICI 118630 was found to increase testosterone production in purified rat testis Leydig cells in a concentration- and time-dependent manner, but no consistent changes in cyclic AMP levels were detectable. The stimulation of steroidogenesis by LHRH agonist was found to be dependent on the concentration of Ca2+ in the incubation medium; at least 1 mM was required. The calcium ionophore A23187 mimicked the effects of the LHRH agonist on steroidogenesis, and addition of both compounds together did not further increase testosterone production. The calcium ionophore caused a small increase in cyclic AMP which was independent of the concentration of the ionophore and of the calcium concentrations. The evidence obtained in this study indicates that LHRH agonist-stimulated steroidogenesis in rat testis Leydig cells is primarily mediated by calcium and not cyclic AMP.
摘要
促黄体生成激素释放激素（LHRH）激动剂ICI 118630被发现以浓度和时间依赖性方式增加纯化的大鼠睾丸间质细胞中的睾酮生成，但未检测到环磷酸腺苷（cAMP）水平有一致变化。发现LHRH激动剂对类固醇生成的刺激依赖于孵育培养基中Ca2+的浓度；至少需要1 mM。钙离子载体A23187模拟了LHRH激动剂对类固醇生成的作用，同时添加这两种化合物并不会进一步增加睾酮生成。钙离子载体导致cAMP有小幅增加，这与离子载体的浓度和钙浓度无关。本研究获得的证据表明，LHRH激动剂刺激大鼠睾丸间质细胞中的类固醇生成主要由钙介导，而非cAMP。
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The role of calcium in luteinizing hormone-releasing hormone agonist (ICI 118630)-stimulated steroidogenesis in rat Leydig cells.钙在促黄体生成激素释放激素激动剂（ICI 118630）刺激大鼠睾丸间质细胞类固醇生成中的作用。
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本文引用的文献
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Stimulation of Leydig cell testosterone secretion in vitro and in vivo in hypophysectomized rats by an agonist of luteinizing hormone releasing hormone.促黄体生成素释放激素激动剂对去垂体大鼠体外和体内睾丸间质细胞睾酮分泌的刺激作用
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Stimulation of progesterone and prostaglandin E accumulation by luteinizing hormone-releasing hormone (LHRH) and LHRH analogs in rat granulosa cells.促黄体生成激素释放激素（LHRH）及LHRH类似物对大鼠颗粒细胞孕酮和前列腺素E蓄积的刺激作用。
Endocrinology. 1982 Jan;110(1):146-52. doi: 10.1210/endo-110-1-146.
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Luteinizing hormone releasing hormone agonists induce ovulation in hypophysectomized proestrous rats: direct ovarian effect.促黄体生成素释放激素激动剂可诱导垂体切除的动情前期大鼠排卵：直接的卵巢效应。
Life Sci. 1981 Jul 13;29(2):185-92. doi: 10.1016/0024-3205(81)90287-3.
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Mechanism of action of gonadotropin-releasing hormone. Involvement of phospholipid turnover in luteinizing hormone release.促性腺激素释放激素的作用机制。磷脂周转在促黄体生成素释放中的作用。
J Biol Chem. 1981 Mar 10;256(5):2226-9.
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10
Stimulation and inhibition by LHRH analogues of cultured rat Leydig cell function and lack of effect on mouse Leydig cells.促黄体生成素释放激素类似物对培养的大鼠睾丸间质细胞功能的刺激和抑制作用以及对小鼠睾丸间质细胞无影响。
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