Aldred L F, Cooke B A
J Steroid Biochem. 1983 Apr;18(4):411-4. doi: 10.1016/0022-4731(83)90059-6.
The ability of cells to exclude pyridine nucleotides was found to be a more sensitive index of cell viability than trypan blue. The three nucleated cell bands obtained by centrifugation of crude rat testis cells on Percoll density gradients contained different proportions of viable and damaged cells. Band III, the steroidogenically active Leydig cell band was mainly viable cells, whilst bands I and II contained 70 and 35% damaged cells respectively. Leydig cells damaged by freezing were shown to have a lower density than viable Leydig cells and did not produce testosterone after stimulation with LH. It is concluded that the methods used to prepare and purify rat testis Leydig cells contribute to the apparent Leydig cell "heterogeneity".
细胞排除吡啶核苷酸的能力被发现是比台盼蓝更敏感的细胞活力指标。通过在Percoll密度梯度上对大鼠睾丸粗细胞进行离心获得的三条有核细胞带含有不同比例的活细胞和受损细胞。带III，即具有类固醇生成活性的莱迪希细胞带主要是活细胞，而带I和带II分别含有70%和35%的受损细胞。经冷冻损伤的莱迪希细胞显示出比活莱迪希细胞更低的密度，并且在用促黄体生成素刺激后不产生睾酮。得出的结论是，用于制备和纯化大鼠睾丸莱迪希细胞的方法导致了明显的莱迪希细胞“异质性”。
