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磷脂酰肌醇4,5 - 二磷酸可能是刺激人血小板后质膜结合钙释放的部位。

Phosphatidylinositol 4,5-bisphosphate may represent the site of release of plasma membrane-bound calcium upon stimulation of human platelets.

作者信息

Broekman M J

出版信息

Biochem Biophys Res Commun. 1984 Apr 16;120(1):226-31. doi: 10.1016/0006-291x(84)91437-2.

DOI:10.1016/0006-291x(84)91437-2
PMID:6324792
Abstract

Thrombin stimulation of human blood platelets caused an extensive (up to 45%) and rapid (5-10 s) decline in endogenous phosphatidylinositol 4,5-bisphosphate (PI-P2). Thrombin initiated an equally rapid loss of membrane-bound Ca, as indicated by the decrease in fluorescence of chlortetracycline (CTC)-loaded platelets. PI-P2 breakdown also correlated with decreased CTC fluorescence upon use of other platelet stimuli: Arachidonate caused moderate and slow decreases in both PI-P2 and CTC fluorescence, while ionophore only induced minimal changes. Thrombin-induced decreases in PI-P2 content could account for release of sufficient membrane-bound Ca to raise cytoplasmic free [Ca2+] to 1-2 microM, supporting the hypothesis that PI-P2 represents the Ca-binding site involved in the stimulus-dependent increase in cytoplasmic Ca2+ evoked by receptor-ligand interactions.

摘要

凝血酶刺激人血小板会导致内源性磷脂酰肌醇4,5-二磷酸（PI-P2）大量（高达45%）且迅速（5 - 10秒）减少。凝血酶引发膜结合钙同样迅速的流失，这通过加载金霉素（CTC）的血小板荧光降低得以表明。使用其他血小板刺激剂时，PI-P2的分解也与CTC荧光降低相关：花生四烯酸导致PI-P2和CTC荧光适度且缓慢降低，而离子载体仅引起微小变化。凝血酶诱导的PI-P2含量降低可解释释放足够的膜结合钙，使细胞质游离[Ca2+]升高至1 - 2微摩尔，支持了PI-P2代表参与受体-配体相互作用引起的细胞质Ca2+刺激依赖性增加的钙结合位点这一假说。

相似文献

1
Phosphatidylinositol 4,5-bisphosphate may represent the site of release of plasma membrane-bound calcium upon stimulation of human platelets.磷脂酰肌醇4,5 - 二磷酸可能是刺激人血小板后质膜结合钙释放的部位。
Biochem Biophys Res Commun. 1984 Apr 16;120(1):226-31. doi: 10.1016/0006-291x(84)91437-2.
2
Rapid decrease of phosphatidylinositol 4,5-bisphosphate in thrombin-stimulated platelets.凝血酶刺激的血小板中磷脂酰肌醇4,5-二磷酸的快速减少。
J Biol Chem. 1982 Nov 10;257(21):12705-8.
3
Hydrolysis of polyphosphoinositides in human platelets.人血小板中多磷酸肌醇的水解
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4
Changes in the platelet phosphoinositides during the first minute after stimulation of washed rabbit platelets with thrombin.用凝血酶刺激洗涤过的兔血小板后第一分钟内血小板磷酸肌醇的变化。
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The decrease in phosphatidylinositol 4,5-bisphosphate in ADP-stimulated washed rabbit platelets is not primarily due to phospholipase C activation.在二磷酸腺苷（ADP）刺激的洗涤兔血小板中，磷脂酰肌醇4,5-二磷酸的减少并非主要由于磷脂酶C的激活。
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6
Serotonin release and phosphoinositide breakdown in thrombin-induced activation of human platelets.凝血酶诱导人血小板活化过程中的5-羟色胺释放与磷酸肌醇分解
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Ca2+ requirement in hydrolysis of phosphatidylinositol-4,5-bisphosphate in human platelets.
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Molecular membrane organization in normal and pathological platelets: changes in inositide metabolism and membrane fluidity.正常和病理状态下血小板的分子膜组织：肌醇磷脂代谢和膜流动性的变化
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Stimulation of phosphate uptake in human platelets by thrombin and collagen. Changes in specific 32P labeling of metabolic ATP and polyphosphoinositides.凝血酶和胶原蛋白对人血小板中磷酸盐摄取的刺激作用。代谢性ATP和多磷酸肌醇特异性32P标记的变化。
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Tight coupling of thrombin-induced acid hydrolase secretion and phosphatidate synthesis to receptor occupancy in human platelets.凝血酶诱导的酸性水解酶分泌和磷脂酸合成与人血小板中受体占据的紧密偶联。
Biochem J. 1984 Aug 15;222(1):157-67. doi: 10.1042/bj2220157.

引用本文的文献

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Inositol-containing lipids in suspension-cultured plant cells: an isotopic study.悬浮培养植物细胞中的含肌醇脂质：一项同位素研究。
Plant Physiol. 1988 May;87(1):217-22. doi: 10.1104/pp.87.1.217.
2
Early transmembrane events in alloimmune cytotoxic T-lymphocyte activation as revealed by stopped-flow fluorometry.停流荧光法揭示的同种免疫细胞毒性T淋巴细胞激活中的早期跨膜事件
Proc Natl Acad Sci U S A. 1986 Mar;83(6):1877-80. doi: 10.1073/pnas.83.6.1877.
3
Effect of common agonists on cytoplasmic ionized calcium concentration in platelets. Measurement with 2-methyl-6-methoxy 8-nitroquinoline (quin2) and aequorin.
常见激动剂对血小板胞质游离钙浓度的影响。用2-甲基-6-甲氧基-8-硝基喹啉（quin2）和水母发光蛋白进行测量。
J Clin Invest. 1986 Mar;77(3):878-86. doi: 10.1172/JCI112385.
4
Inositol cyclic triphosphate [inositol 1,2-(cyclic)-4,5-triphosphate] is formed upon thrombin stimulation of human platelets.肌醇环状三磷酸酯[肌醇1,2-(环状)-4,5-三磷酸酯]在凝血酶刺激人血小板时形成。
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6397-401. doi: 10.1073/pnas.83.17.6397.
5
The role of phosphoinositides in signal transduction.磷酸肌醇在信号转导中的作用。
J Membr Biol. 1986;89(3):193-210. doi: 10.1007/BF01870664.
6
Phorbol myristate acetate inhibits thrombin-stimulated Ca2+ mobilization and phosphatidylinositol 4,5-bisphosphate hydrolysis in human platelets.佛波醇肉豆蔻酸酯乙酸盐可抑制人血小板中凝血酶刺激的Ca2+动员和磷脂酰肌醇4,5-二磷酸水解。
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3859-62. doi: 10.1073/pnas.82.11.3859.
7
1,2-Diacylglycerol and phorbol ester inhibit agonist-induced formation of inositol phosphates in human platelets: possible implications for negative feedback regulation of inositol phospholipid hydrolysis.1,2 - 二酰甘油和佛波酯抑制激动剂诱导的人血小板中肌醇磷酸的形成：对肌醇磷脂水解负反馈调节的可能影响。
Proc Natl Acad Sci U S A. 1985 May;82(9):2623-6. doi: 10.1073/pnas.82.9.2623.
8
Phosphatidylinositol 4,5-bisphosphate is selectively retained by platelet-fibrin clots formed by thrombin.磷脂酰肌醇4,5 - 二磷酸被凝血酶形成的血小板 - 纤维蛋白凝块选择性保留。
Biochem J. 1987 Aug 1;245(3):649-53. doi: 10.1042/bj2450649.
9
Activation of phospholipase C associated with isolated rabbit platelet membranes by guanosine 5'-[gamma-thio]triphosphate and by thrombin in the presence of GTP.在存在鸟苷5'-[γ-硫代]三磷酸（GTP）的情况下，鸟苷5'-[γ-硫代]三磷酸和凝血酶对与兔血小板分离膜相关的磷脂酶C的激活作用。
Biochem J. 1987 Apr 15;243(2):457-65. doi: 10.1042/bj2430457.
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Thrombin induces a biphasic 1,2-diacylglycerol production in human platelets.凝血酶可诱导人血小板产生双相的1,2 - 二酰甘油。
Biochem J. 1991 Apr 15;275 ( Pt 2)(Pt 2):355-61. doi: 10.1042/bj2750355.