来自胚胎小鸡肌肉中乙酰胆碱受体的单通道电流。爆发内间隙的动力学和电导特性。
Single-channel currents from acetylcholine receptors in embryonic chick muscle. Kinetic and conductance properties of gaps within bursts.
作者信息
Auerbach A, Sachs F
出版信息
Biophys J. 1984 Jan;45(1):187-98. doi: 10.1016/S0006-3495(84)84147-8.
Abstract
In tissue-cultured chick muscle, bursts of current from single nicotinic ion channels contain a variety of low-conductance gaps. One population has a lifetime of approximately 0.1 ms and an unknown conductance. A second population has a lifetime of 2-10 ms and conductance of zero. The third population has a lifetime of 0.5-1 ms and a mean conductance approximately 2% that of the main conductance state. This subconductance state has an agonist-dependent lifetime, longer for suberyldicholine than for acetylcholine, and is liganded to the same extent as the main conductance state. Subconductance gaps have a linear current-voltage behavior in the range -60 to -140 mV and appear to have the same reversal potential as the main state. The subconductance state is composed of a group of states which interconvert with correlation times longer than 300 microseconds.
摘要
在组织培养的鸡肌肉中，单个烟碱型离子通道的电流猝发包含多种低电导间隙。一类间隙的寿命约为0.1毫秒，电导未知。另一类间隙的寿命为2 - 10毫秒，电导为零。第三类间隙的寿命为0.5 - 1毫秒，平均电导约为主电导状态的2%。这种亚电导状态具有依赖激动剂的寿命，对于辛二酰胆碱而言比对乙酰胆碱更长，并且与主电导状态的配体结合程度相同。亚电导间隙在-60至-140毫伏范围内具有线性电流-电压行为，并且似乎与主状态具有相同的反转电位。亚电导状态由一组相互转换的状态组成，其相关时间超过300微秒。
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