肌肉烟碱受体通道的电导在门控时迅速变化。
The conductance of the muscle nicotinic receptor channel changes rapidly upon gating.
作者信息
Maconochie D J, Fletcher G H, Steinbach J H
机构信息
Department of Anesthesiology, Washington University School of Medicine, St. Louis, Missouri 63110.
出版信息
Biophys J. 1995 Feb;68(2):483-90. doi: 10.1016/S0006-3495(95)80209-2.
Abstract
We have recorded single-channel currents through fetal-type muscle nicotinic receptor channels at recording bandwidths of approximately 50 and 75 kHz. The time course of the rising phase of aligned and averaged openings can be entirely accounted for if it is assumed that the conductance of the single channel changes instantaneously, and that alignment and averaging introduce a dispersion of 2-3 microseconds. We conclude that we find no evidence for a gradual change in conductance as a channel opens or closes. The shapes of averaged power spectra are consistent with this conclusion, insofar as they exclude an exponential relaxation in the transition with a time constant of 10 microseconds or more.
摘要
我们在大约50和75千赫兹的记录带宽下记录了通过胎儿型肌肉烟碱受体通道的单通道电流。如果假设单通道电导瞬间变化，并且对齐和平均引入2-3微秒的离散，那么对齐和平均后的开放上升相的时间进程可以得到完全解释。我们得出结论，没有发现通道打开或关闭时电导逐渐变化的证据。平均功率谱的形状与这一结论一致，因为它们排除了具有10微秒或更长时间常数的跃迁中的指数弛豫。
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