β-肾上腺素能细胞表面受体的快速可逆性消失
Rapid and reversible disappearance of beta-adrenergic cell surface receptors.
作者信息
Staehelin M, Simons P
出版信息
EMBO J. 1982;1(2):187-90. doi: 10.1002/j.1460-2075.1982.tb01145.x.
Abstract
The water soluble beta-adrenergic ligand [3H]CGP-12177 was used to measure the cell surface receptors in intact cells. In two cell lines, C6 glioma and WEHI 7 lymphoma cells, -50% of the cell surface receptors disappear within minutes of incubation of the cells with isoproterenol. The receptors can still be detected in homogenates and reappear on the cell surface when cells are washed and reincubated at 37 degrees C. The data agree with a disappearance of the receptors from the cell surface by an agonist-mediated endocytosis.
摘要
水溶性β-肾上腺素能配体[3H]CGP - 12177用于测量完整细胞中的细胞表面受体。在两种细胞系，即C6胶质瘤细胞和WEHI 7淋巴瘤细胞中，细胞与异丙肾上腺素孵育数分钟内，约50%的细胞表面受体消失。在匀浆中仍可检测到这些受体，当细胞洗涤后在37℃再孵育时，受体又重新出现在细胞表面。这些数据与激动剂介导的内吞作用导致受体从细胞表面消失的情况相符。
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