异丙肾上腺素处理后完整C6细胞中β-肾上腺素能受体的重现。
Reappearance of beta-adrenergic receptors after isoproterenol treatment in intact C6-cells.
作者信息
Hertel C, Staehelin M
出版信息
J Cell Biol. 1983 Nov;97(5 Pt 1):1538-43. doi: 10.1083/jcb.97.5.1538.
Abstract
The reappearance of beta-adrenergic receptors in C6-glioma cells after desensitization with isoproterenol was studied using the antagonist [3H]CGP-12177. Reappearance had the following properties: (a) it occurred in intact cells only, (b) it was temperature dependent, (c) it required an Na+/H+ gradient, low intracellular Ca2+ activity, and (d) it required ATP, and (e) intact lysosomes. The results suggest endocytosis and recycling of the beta-adrenergic receptor after agonist treatment.
摘要
使用拮抗剂[3H]CGP - 12177研究了异丙肾上腺素脱敏后C6胶质瘤细胞中β - 肾上腺素能受体的重新出现情况。重新出现具有以下特性：(a) 仅在完整细胞中发生，(b) 与温度有关，(c) 需要Na+/H+梯度、低细胞内Ca2+活性，以及(d) 需要ATP，和(e) 完整的溶酶体。结果表明激动剂处理后β - 肾上腺素能受体发生了内吞和再循环。
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