Lichtenberg V, Weise H C, Graesslin D, Bettendorf G
FEBS Lett. 1984 Apr 9;169(1):21-4. doi: 10.1016/0014-5793(84)80281-1.
The effect of the 7 different molecular forms of human pituitary lutropin (LH) on mouse Leydig cells as a model of a target organ were studied. The hormone functions have been characterized by receptor binding, cAMP accumulation and testosterone production. One important finding was the similar intrinsic in vitro biological activity for all isohormones. Quantitatively, however, the potencies of the 7 hormone forms did not correlate with the activities obtained by radioimmunoassay: there was a dramatic decrease of receptor binding activity and biological activity compared to immunoactivity from the more alkaline to the more acidic LH isohormones.
研究了7种不同分子形式的人垂体促黄体生成素（LH）对作为靶器官模型的小鼠睾丸间质细胞的影响。通过受体结合、环磷酸腺苷（cAMP）积累和睾酮生成来表征激素功能。一个重要发现是所有同工激素在体外具有相似的内在生物活性。然而，从数量上看，这7种激素形式的效力与放射免疫测定所获得的活性并不相关：与免疫活性相比，从碱性更强到酸性更强的LH同工激素，其受体结合活性和生物活性显著降低。
