Wikström M
FEBS Lett. 1984 Apr 24;169(2):300-4. doi: 10.1016/0014-5793(84)80338-5.
The spectrophotometric indicators neutral red and safranine were used to determine the relative H+/e- ratios of proton uptake from the mitochondrial matrix, and the q+/e- ratios of electrical charge translocation, during oxidation of beta-OH-butyrate and succinate by ferricyanide in rat liver mitochondria. With beta-OH-butyrate both ratios were higher than with succinate by a factor close to 3.0. Since there is full agreement that H+/e- of proton uptake and q+/e- of charge translocation are both equal to unity for oxidation of succinate (or ubiquinol) by ferricytochrome c, the corresponding ratios for oxidation of NADH by ubiquinone and cytochrome c are near 2.0 and 3.0, respectively.
在大鼠肝线粒体中，利用分光光度法指示剂中性红和番红，测定了在铁氰化物氧化β-羟基丁酸酯和琥珀酸的过程中，从线粒体基质摄取质子的相对H⁺/e⁻比率以及电荷转移的q⁺/e⁻比率。对于β-羟基丁酸酯，这两个比率均高于琥珀酸，其倍数接近3.0。由于完全一致的观点认为，对于铁细胞色素c氧化琥珀酸（或泛醇）而言，摄取质子的H⁺/e⁻和电荷转移的q⁺/e⁻均等于1，因此泛醌和细胞色素c氧化NADH的相应比率分别接近2.0和3.0。
