牛肝中抑制性丝氨酰 - tRNA：ATP磷酸转移酶的纯化及性质

Purification and properties of suppressor seryl-tRNA: ATP phosphotransferase from bovine liver.

作者信息

Mizutani T, Hashimoto A

出版信息

FEBS Lett. 1984 Apr 24;169(2):319-22. doi: 10.1016/0014-5793(84)80342-7.

Abstract

Seryl-tRNASerCmCA: ATP phosphotransferase was purified 1200-fold from bovine liver by ultracentrifugation at 150 000 X g, chromatography on DEAE-cellulose, fractional precipitation with ammonium sulfate, chromatography on hydroxyapatite, gel filtration on Sephacryl S-300 and affinity chromatography on Blue Sepharose. Molecular mass was estimated as 135-145 kDa. The Km values for ATP and ser-tRNASerCmCA were 2 mM and 21 nM, respectively. This enzyme did not react with ser-tRNASerIGA, tyr-tRNA or thr-tRNA.

摘要

丝氨酰 - tRNASerCmCA：ATP磷酸转移酶通过在150000×g下超速离心、DEAE - 纤维素柱层析、硫酸铵分级沉淀、羟基磷灰石柱层析、Sephacryl S - 300凝胶过滤以及Blue Sepharose亲和层析从牛肝中纯化了1200倍。估计其分子量为135 - 145 kDa。ATP和丝氨酰 - tRNASerCmCA的Km值分别为2 mM和21 nM。该酶不与丝氨酰 - tRNASerIGA、酪氨酰 - tRNA或苏氨酰 - tRNA反应。