• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

真核生物硒代半胱氨酸插入tRNA种类支持大肠杆菌中的硒蛋白合成。

Eukaryotic selenocysteine inserting tRNA species support selenoprotein synthesis in Escherichia coli.

作者信息

Baron C, Sturchler C, Wu X Q, Gross H J, Krol A, Böck A

机构信息

Lehrstuhl für Mikrobiologie, Universität München, Germany.

出版信息

Nucleic Acids Res. 1994 Jun 25;22(12):2228-33. doi: 10.1093/nar/22.12.2228.

DOI:10.1093/nar/22.12.2228
PMID:8036149
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC523678/
Abstract

Although the tRNA species directing selenocysteine insertion in prokaryotes differ greatly in their primary structure from that of their eukaryotic homologues they share very similar three-dimensional structures. To analyse whether this conservation of the overall shape of the molecules reflects a conservation of their functional interactions it was tested whether the selenocysteine inserting tRNA species from Homo sapiens supports selenoprotein synthesis in E. coli. It was found that the expression of the human tRNA(Sec) gene in E.coli can complement a lesion in the tRNA(Sec) gene of this organism. Transcripts of the Homo sapiens and Xenopus laevis tRNA(Sec) genes synthesised in vitro were amino-acylated by the E.coli seryl-tRNA ligase although at a very low rate and the resulting seryl-tRNA(Sec) was bound to and converted into selenocysteyl-tRNA(Sec) by the selenocysteine synthase of this organism. Selenocysteyl-tRNA(Sec) from both eukaryotes was able to form a complex with translation factor SELB from E.coli. Although the mechanism of selenocysteine incorporation into seleno-proteins appears to be rather different in E.coli and in vertebrates, we observe here a surprising conservation of functions over an enormous evolutionary distance.

摘要

尽管在原核生物中指导硒代半胱氨酸插入的tRNA种类在一级结构上与真核生物的同源物有很大差异，但它们具有非常相似的三维结构。为了分析分子整体形状的这种保守性是否反映了其功能相互作用的保守性，研究人员测试了来自智人的硒代半胱氨酸插入tRNA种类是否支持大肠杆菌中的硒蛋白合成。结果发现，人tRNA(Sec)基因在大肠杆菌中的表达可以弥补该生物体tRNA(Sec)基因的缺陷。在体外合成的智人和非洲爪蟾tRNA(Sec)基因的转录本被大肠杆菌丝氨酰-tRNA连接酶氨酰化，尽管速率非常低，并且产生的丝氨酰-tRNA(Sec)被该生物体的硒代半胱氨酸合酶结合并转化为硒代半胱氨酰-tRNA(Sec)。来自两种真核生物的硒代半胱氨酰-tRNA(Sec)都能够与大肠杆菌的翻译因子SELB形成复合物。尽管在大肠杆菌和脊椎动物中，硒代半胱氨酸掺入硒蛋白的机制似乎有很大不同，但我们在此观察到，在巨大的进化距离上，功能存在惊人的保守性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fefb/523678/8a28539c8084/nar00036-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fefb/523678/29f1a1595323/nar00036-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fefb/523678/8a28539c8084/nar00036-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fefb/523678/29f1a1595323/nar00036-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fefb/523678/8a28539c8084/nar00036-0058-a.jpg

相似文献

1
Eukaryotic selenocysteine inserting tRNA species support selenoprotein synthesis in Escherichia coli.真核生物硒代半胱氨酸插入tRNA种类支持大肠杆菌中的硒蛋白合成。
Nucleic Acids Res. 1994 Jun 25;22(12):2228-33. doi: 10.1093/nar/22.12.2228.
2
The length of the aminoacyl-acceptor stem of the selenocysteine-specific tRNA(Sec) of Escherichia coli is the determinant for binding to elongation factors SELB or Tu.大肠杆菌中硒代半胱氨酸特异性转运RNA（Sec）的氨酰基-受体茎的长度是其与延伸因子SELB或Tu结合的决定因素。
J Biol Chem. 1991 Oct 25;266(30):20375-9.
3
A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro.一种用于EF-Tu介导的硒代半胱氨酸在体内和体外掺入的合成tRNA。
FEBS Lett. 2015 Aug 4;589(17):2194-9. doi: 10.1016/j.febslet.2015.06.039. Epub 2015 Jul 6.
4
Recognition of the mRNA selenocysteine insertion sequence by the specialized translational elongation factor SELB.特殊翻译延伸因子SELB对mRNA硒代半胱氨酸插入序列的识别。
Genes Dev. 1994 Feb 1;8(3):376-85. doi: 10.1101/gad.8.3.376.
5
Genes coding for the selenocysteine-inserting tRNA species from Desulfomicrobium baculatum and Clostridium thermoaceticum: structural and evolutionary implications.编码来自杆状脱硫微菌和热乙酸梭菌的硒代半胱氨酸插入tRNA种类的基因：结构和进化意义
J Bacteriol. 1994 Mar;176(5):1268-74. doi: 10.1128/jb.176.5.1268-1274.1994.
6
High-level expression in Escherichia coli of selenocysteine-containing rat thioredoxin reductase utilizing gene fusions with engineered bacterial-type SECIS elements and co-expression with the selA, selB and selC genes.利用与工程化细菌型硒代半胱氨酸插入序列元件的基因融合以及与selA、selB和selC基因共表达，在大肠杆菌中实现含硒代半胱氨酸的大鼠硫氧还蛋白还原酶的高水平表达。
J Mol Biol. 1999 Oct 8;292(5):1003-16. doi: 10.1006/jmbi.1999.3085.
7
Selenocysteine tRNAs as central components of selenoprotein biosynthesis in eukaryotes.硒代半胱氨酸tRNA作为真核生物中硒蛋白生物合成的核心组成部分。
Biomed Environ Sci. 1997 Sep;10(2-3):116-24.
8
A G.U base pair in the eukaryotic selenocysteine tRNA is important for interaction with SePF, the putative selenocysteine-specific elongation factor.真核生物硒代半胱氨酸转运RNA中的一个鸟嘌呤-尿嘧啶碱基对对于与SePF（一种假定的硒代半胱氨酸特异性延伸因子）的相互作用很重要。
FEBS Lett. 1998 Jun 12;429(2):189-93. doi: 10.1016/s0014-5793(98)00589-4.
9
The Specific Elongation Factor to Selenocysteine Incorporation in Escherichia coli: Unique tRNA Recognition and its Interactions.硒代半胱氨酸掺入大肠杆菌的特异延伸因子：独特的 tRNA 识别及其相互作用。
J Mol Biol. 2021 Nov 19;433(23):167279. doi: 10.1016/j.jmb.2021.167279. Epub 2021 Oct 5.
10
The function of selenocysteine synthase and SELB in the synthesis and incorporation of selenocysteine.硒代半胱氨酸合成酶和SELB在硒代半胱氨酸合成与掺入过程中的作用。
Biochimie. 1991 Dec;73(12):1481-6. doi: 10.1016/0300-9084(91)90181-y.

引用本文的文献

1
Selenocysteine tRNA, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification, and Sequencing.硒代半胱氨酸转运RNA，硒蛋白生物合成的核心组成部分：分离、鉴定、修饰及测序
Methods Mol Biol. 2018;1661:43-60. doi: 10.1007/978-1-4939-7258-6_4.
2
Tertiary structure of bacterial selenocysteine tRNA.细菌硒代半胱氨酸 tRNA 的三级结构。
Nucleic Acids Res. 2013 Jul;41(13):6729-38. doi: 10.1093/nar/gkt321. Epub 2013 May 6.
3
Amino acid modifications on tRNA.转运核糖核酸（tRNA）上的氨基酸修饰

本文引用的文献

1
Solution structure of selenocysteine-inserting tRNA(Sec) from Escherichia coli. Comparison with canonical tRNA(Ser).来自大肠杆菌的插入硒代半胱氨酸的tRNA（Sec）的溶液结构。与典型tRNA（Ser）的比较。
J Mol Biol. 1993 May 20;231(2):274-92. doi: 10.1006/jmbi.1993.1282.
2
Interaction of translation factor SELB with the formate dehydrogenase H selenopolypeptide mRNA.翻译因子SELB与甲酸脱氢酶H硒代多肽mRNA的相互作用。
Proc Natl Acad Sci U S A. 1993 May 1;90(9):4181-5. doi: 10.1073/pnas.90.9.4181.
3
Unique secondary and tertiary structural features of the eucaryotic selenocysteine tRNA(Sec).
Acta Biochim Biophys Sin (Shanghai). 2008 Jul;40(7):539-53. doi: 10.1111/j.1745-7270.2008.00435.x.
4
From one amino acid to another: tRNA-dependent amino acid biosynthesis.从一种氨基酸到另一种氨基酸：依赖于转运RNA的氨基酸生物合成
Nucleic Acids Res. 2008 Apr;36(6):1813-25. doi: 10.1093/nar/gkn015. Epub 2008 Feb 5.
5
Selenocysteine tRNA-specific elongation factor SelB is a structural chimaera of elongation and initiation factors.硒代半胱氨酸tRNA特异性延伸因子SelB是延伸因子和起始因子的结构嵌合体。
EMBO J. 2005 Jan 12;24(1):11-22. doi: 10.1038/sj.emboj.7600505. Epub 2004 Dec 23.
6
Barriers to heterologous expression of a selenoprotein gene in bacteria.细菌中硒蛋白基因异源表达的障碍。
J Bacteriol. 1997 Feb;179(3):576-82. doi: 10.1128/jb.179.3.576-582.1997.
真核生物硒代半胱氨酸转运RNA（Sec）独特的二级和三级结构特征。
Nucleic Acids Res. 1993 Mar 11;21(5):1073-9. doi: 10.1093/nar/21.5.1073.
4
Conserved nucleotide sequences in the open reading frame and 3' untranslated region of selenoprotein P mRNA.硒蛋白P信使核糖核酸开放阅读框和3'非翻译区中的保守核苷酸序列。
Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):537-41. doi: 10.1073/pnas.90.2.537.
5
Identity determinants of human tRNA(Ser): sequence elements necessary for serylation and maturation of a tRNA with a long extra arm.人tRNA（Ser）的身份决定因素：具有长额外臂的tRNA进行丝氨酸化和成熟所必需的序列元件。
EMBO J. 1993 Aug;12(8):3333-8. doi: 10.1002/j.1460-2075.1993.tb06003.x.
6
Functional characterization of the eukaryotic SECIS elements which direct selenocysteine insertion at UGA codons.指导硒代半胱氨酸在UGA密码子处插入的真核生物SECIS元件的功能特性。
EMBO J. 1993 Aug;12(8):3315-22. doi: 10.1002/j.1460-2075.1993.tb06001.x.
7
Dietary selenium affects methylation of the wobble nucleoside in the anticodon of selenocysteine tRNA([Ser]Sec).膳食硒会影响硒代半胱氨酸tRNA（[Ser]Sec）反密码子中摆动核苷的甲基化。
J Biol Chem. 1993 Jul 5;268(19):14215-23.
8
The length and the secondary structure of the D-stem of human selenocysteine tRNA are the major identity determinants for serine phosphorylation.人硒代半胱氨酸tRNA的D茎的长度和二级结构是丝氨酸磷酸化的主要识别决定因素。
EMBO J. 1994 Jan 1;13(1):241-8. doi: 10.1002/j.1460-2075.1994.tb06254.x.
9
The long extra arms of human tRNA((Ser)Sec) and tRNA(Ser) function as major identify elements for serylation in an orientation-dependent, but not sequence-specific manner.人tRNA（（Ser）Sec）和tRNA（Ser）的长额外臂作为丝氨酰化的主要识别元件，以方向依赖但非序列特异性的方式起作用。
Nucleic Acids Res. 1993 Dec 11;21(24):5589-94. doi: 10.1093/nar/21.24.5589.
10
Effect of the relative position of the UGA codon to the unique secondary structure in the fdhF mRNA on its decoding by selenocysteinyl tRNA in Escherichia coli.fdhF信使核糖核酸中UGA密码子相对于独特二级结构的相对位置对其在大肠杆菌中被硒代半胱氨酰转运核糖核酸解码的影响。
J Biol Chem. 1993 Nov 5;268(31):23128-31.