用于检测马传染性贫血病毒p26抗原和抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for detection of equine infectious anemia virus p26 antigen and antibody.
作者信息
Shane B S, Issel C J, Montelaro R C
出版信息
J Clin Microbiol. 1984 Mar;19(3):351-5. doi: 10.1128/jcm.19.3.351-355.1984.
Abstract
A sensitive specific enzyme-linked immunosorbent assay utilizing purified p26 antigen was developed for the detection of antibodies to equine infectious anemia virus in naturally and experimentally infected horses. Generally, antibodies to the virus could be detected by the enzyme-linked immunosorbent assay 3 to 4 days earlier than by the standard agar gel immunodiffusion test, and they could be detected more reliably in horses with weak or equivocal agar gel immunodiffusion test reactions. The enzyme-linked immunosorbent assay was also successfully applied to the detection of p26 antigen in tissue culture fluids.
摘要
利用纯化的p26抗原开发了一种灵敏特异的酶联免疫吸附测定法，用于检测自然感染和实验感染马匹中抗马传染性贫血病毒的抗体。一般来说，通过酶联免疫吸附测定法检测病毒抗体比标准琼脂凝胶免疫扩散试验早3至4天，并且在琼脂凝胶免疫扩散试验反应微弱或不明确的马匹中能更可靠地检测到抗体。酶联免疫吸附测定法也成功应用于检测组织培养液中的p26抗原。
相似文献
1
Enzyme-linked immunosorbent assay for detection of equine infectious anemia virus p26 antigen and antibody.用于检测马传染性贫血病毒p26抗原和抗体的酶联免疫吸附测定。
J Clin Microbiol. 1984 Mar;19(3):351-5. doi: 10.1128/jcm.19.3.351-355.1984.
2
3
Evaluation of equine infectious anemia virus core proteins produced in a baculovirus expression system in agar gel immunodiffusion test and enzyme-linked immunosorbent assay.在琼脂凝胶免疫扩散试验和酶联免疫吸附测定中对杆状病毒表达系统产生的马传染性贫血病毒核心蛋白的评估。
J Vet Med Sci. 1998 Dec;60(12):1361-2. doi: 10.1292/jvms.60.1361.
4
Western blot assay using recombinant p26 antigen for detection of equine infectious anemia virus-specific antibodies.使用重组p26抗原的蛋白质印迹分析检测马传染性贫血病毒特异性抗体。
Clin Vaccine Immunol. 2007 Dec;14(12):1646-8. doi: 10.1128/CVI.00293-07. Epub 2007 Oct 24.
5
Application of equine infectious anemia virus core proteins produced in a baculovirus expression system to serological diagnosis.杆状病毒表达系统产生的马传染性贫血病毒核心蛋白在血清学诊断中的应用
Microbiol Immunol. 1997;41(12):975-80. doi: 10.1111/j.1348-0421.1997.tb01957.x.
6
Development of an enzyme-linked immunosorbent assay for equine infectious anemia virus detection using recombinant Pr55gag.利用重组Pr55gag开发用于检测马传染性贫血病毒的酶联免疫吸附测定法。
J Clin Microbiol. 1989 Jun;27(6):1167-73. doi: 10.1128/jcm.27.6.1167-1173.1989.
7
Enzyme-linked immunosorbent assay for diagnosis of equine infectious anemia.用于诊断马传染性贫血的酶联免疫吸附测定
Vet Microbiol. 1982 Sep;7(4):307-15. doi: 10.1016/0378-1135(82)90010-4.
8
9
10
Enzyme-linked immunosorbent assay and agar gel immunodiffusion assay for diagnosis of equine infectious anemia employing p26 protein fused to the maltose-binding protein.酶联免疫吸附试验和琼脂凝胶免疫扩散试验诊断马传染性贫血，采用融合麦芽糖结合蛋白的 p26 蛋白。
Arch Virol. 2018 Oct;163(10):2871-2875. doi: 10.1007/s00705-018-3923-6. Epub 2018 Jul 7.
引用本文的文献
1
Development of a colloidal gold immunochromatographic strip to detect equine infectious anemia virus.用于检测马传染性贫血病毒的胶体金免疫层析试纸条的研制
Virol J. 2025 Jun 24;22(1):205. doi: 10.1186/s12985-025-02815-6.
2
Development, evaluation, and laboratory validation of immunoassays for the diagnosis of equine infectious anemia (EIA) using recombinant protein produced from a synthetic p26 gene of EIA virus.利用从马传染性贫血病毒（EIA）合成p26基因产生的重组蛋白开发、评估和实验室验证用于诊断马传染性贫血（EIA）的免疫测定法。
Indian J Virol. 2013 Dec;24(3):349-56. doi: 10.1007/s13337-013-0149-9. Epub 2013 Aug 8.
3
4
Serological method using recombinant S2 protein to differentiate equine infectious anemia virus (EIAV)-infected and EIAV-vaccinated horses.使用重组S2蛋白的血清学方法区分感染马传染性贫血病毒（EIAV）和接种EIAV疫苗的马匹。
Clin Diagn Lab Immunol. 2004 Nov;11(6):1120-9. doi: 10.1128/CDLI.11.6.1120-1129.2004.
5
Equine infectious anemia.马传染性贫血
Vet Clin North Am Equine Pract. 1993 Aug;9(2):321-36. doi: 10.1016/s0749-0739(17)30399-1.
6
The immunopathogenesis of equine infectious anemia virus.马传染性贫血病毒的免疫发病机制。
Virus Res. 1994 May;32(2):111-38. doi: 10.1016/0168-1702(94)90038-8.
7
Use of an ELISA test in the eradication of an equine infectious anaemia focus.酶联免疫吸附测定法在根除马传染性贫血疫点中的应用。
Trop Anim Health Prod. 1994 May;26(2):65-8. doi: 10.1007/BF02239899.
8
Rapid emergence of novel antigenic and genetic variants of equine infectious anemia virus during persistent infection.马传染性贫血病毒持续感染期间新型抗原和基因变异体的快速出现。
J Virol. 1986 Jan;57(1):71-80. doi: 10.1128/JVI.57.1.71-80.1986.
9
Development of an enzyme-linked immunosorbent assay for equine infectious anemia virus detection using recombinant Pr55gag.利用重组Pr55gag开发用于检测马传染性贫血病毒的酶联免疫吸附测定法。
J Clin Microbiol. 1989 Jun;27(6):1167-73. doi: 10.1128/jcm.27.6.1167-1173.1989.
10
Analysis of equine humoral immune responses to the transmembrane envelope glycoprotein (gp45) of equine infectious anemia virus.马对马传染性贫血病毒跨膜包膜糖蛋白（gp45）的体液免疫反应分析。
J Virol. 1991 Feb;65(2):1013-8. doi: 10.1128/JVI.65.2.1013-1018.1991.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The micro-ELISA sandwich technique for the quantitation of Leishmania donovani soluble antigen.
Ann Trop Med Parasitol. 1980 Dec;74(6):591-5. doi: 10.1080/00034983.1980.11687392.
3
Enzyme-linked immunosorbent assay for diagnosis of equine infectious anemia.用于诊断马传染性贫血的酶联免疫吸附测定
Vet Microbiol. 1982 Sep;7(4):307-15. doi: 10.1016/0378-1135(82)90010-4.
4
5
Cytomegalovirus antibody in cerebrospinal fluid of schizophrenic patients detected by enzyme immunoassay.采用酶免疫测定法检测精神分裂症患者脑脊液中的巨细胞病毒抗体。
Science. 1982 May 21;216(4548):892-4. doi: 10.1126/science.6281883.
6
Detection of equine infectious anemia virus in a horse with an equivocal agar gel immunodiffusion test reaction.在一匹琼脂凝胶免疫扩散试验反应不明确的马中检测马传染性贫血病毒。
J Am Vet Med Assoc. 1982 Feb 1;180(3):276-8.
7
Evaluation of the parameters of the ELISA procedure for feline type C retroviral antigens: assay of FeLV antigens in chronically infected or nonproducer transformed cells.
Intervirology. 1981;15(1):1-9. doi: 10.1159/000149208.
8
An interchangeable ELISA for cytomegalovirus antigen and antibody.一种用于巨细胞病毒抗原和抗体的可互换酶联免疫吸附测定法。
J Virol Methods. 1981 Apr;2(5):301-12. doi: 10.1016/0166-0934(81)90029-x.
9
Equine infectious anemia virus, a putative lentivirus, contains polypeptides analogous to prototype-C oncornaviruses.马传染性贫血病毒，一种假定的慢病毒，含有与原型C型肿瘤病毒类似的多肽。
Virology. 1980 Dec;107(2):520-5. doi: 10.1016/0042-6822(80)90319-0.
10
An enzyme-linked immunosorbent assay for enzootic bovine leukosis virus antibodies.
Vet Rec. 1980 Aug 9;107(6):124-6. doi: 10.1136/vr.107.6.124.
Zotero 插件