用于检测马传染性贫血病毒p26抗原和抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for detection of equine infectious anemia virus p26 antigen and antibody.
作者信息
Shane B S, Issel C J, Montelaro R C
出版信息
J Clin Microbiol. 1984 Mar;19(3):351-5. doi: 10.1128/jcm.19.3.351-355.1984.
Abstract
A sensitive specific enzyme-linked immunosorbent assay utilizing purified p26 antigen was developed for the detection of antibodies to equine infectious anemia virus in naturally and experimentally infected horses. Generally, antibodies to the virus could be detected by the enzyme-linked immunosorbent assay 3 to 4 days earlier than by the standard agar gel immunodiffusion test, and they could be detected more reliably in horses with weak or equivocal agar gel immunodiffusion test reactions. The enzyme-linked immunosorbent assay was also successfully applied to the detection of p26 antigen in tissue culture fluids.
摘要
利用纯化的p26抗原开发了一种灵敏特异的酶联免疫吸附测定法，用于检测自然感染和实验感染马匹中抗马传染性贫血病毒的抗体。一般来说，通过酶联免疫吸附测定法检测病毒抗体比标准琼脂凝胶免疫扩散试验早3至4天，并且在琼脂凝胶免疫扩散试验反应微弱或不明确的马匹中能更可靠地检测到抗体。酶联免疫吸附测定法也成功应用于检测组织培养液中的p26抗原。
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