单克隆抗体证明人胶原酶之间存在异质性，该单克隆抗体可选择性识别并抑制人中性粒细胞胶原酶。
Heterogeneity among human collagenases demonstrated by monoclonal antibody that selectively recognizes and inhibits human neutrophil collagenase.
作者信息
Hasty K A, Hibbs M S, Kang A H, Mainardi C L
出版信息
J Exp Med. 1984 May 1;159(5):1455-63. doi: 10.1084/jem.159.5.1455.
Abstract
The heterogeneity of human collagenases has been examined using a monoclonal antibody to neutrophil collagenase. This antibody inhibited collagenase activity and, when covalently coupled to Sepharose, bound both latent and active enzyme. Although human neutrophil collagenase was inhibited by the antibody, the activity of human skin and rheumatoid synovial collagenase was not significantly diminished in the presence of the antibody. Competitive inhibition studies also differentiated between these collagenases. Only human neutrophil collagenase effectively blocked the antibody in a competitive enzyme-linked immunosorbent assay while skin and rheumatoid synovial collagenase again failed to interact with the antibody. The unequivocal recognition of neutrophil collagenase as an immunologically distinct entity from other collagenases supports the hypothesis that neutrophil collagenase is a separate gene product from fibroblast or synovial collagenase.
摘要
利用抗中性粒细胞胶原酶的单克隆抗体对人胶原酶的异质性进行了研究。该抗体抑制胶原酶活性，当与琼脂糖共价偶联时，能结合潜伏态和活性态的酶。虽然人中性粒细胞胶原酶受到该抗体的抑制，但在有抗体存在的情况下，人皮肤和类风湿性滑膜胶原酶的活性并未显著降低。竞争性抑制研究也区分了这些胶原酶。在竞争性酶联免疫吸附测定中，只有人中性粒细胞胶原酶能有效阻断该抗体，而皮肤和类风湿性滑膜胶原酶再次未能与该抗体相互作用。中性粒细胞胶原酶作为一种在免疫学上与其他胶原酶不同的独特实体得到明确识别，这支持了中性粒细胞胶原酶是成纤维细胞或滑膜胶原酶之外的另一种基因产物的假说。
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