Hasty D L, Beachey E H, Simpson W A, Dale J B
J Exp Med. 1982 Apr 1;155(4):1010-8. doi: 10.1084/jem.155.4.1010.
Hybridoma technology was used to produce a set of monoclonal antibodies against a purified polypeptide fragment of type 24 streptococcal M protein to delineate the protective determinants of M protein exposed on the surface of the virulent streptococci. Several hybridoma antibodies were found to be opsonic against the homologous type streptococci. At least two of these antibodies (IIC3.7 and IIC4.6) protected mice against challenge infections with the homologous, but not a heterologous, serotype of bacteria. One of the hybridoma antibodies that reacted in high dilution (1:204,800) with the isolated M protein failed to react with the M protein on the surface of type 24 streptococci, and thus did not opsonize the homologous organisms or protect mice against challenge infections. Because hybridoma antibodies are directed against a single distinct immunodeterminant, these results indicate that protective immunity may be directed at any one of several distinct antigenic determinants of M protein exposed on the surface of virulent group A streptococci.
采用杂交瘤技术制备了一组针对24型链球菌M蛋白纯化多肽片段的单克隆抗体，以确定暴露在强毒株表面的M蛋白的保护性决定簇。发现几种杂交瘤抗体对同源型链球菌具有调理作用。其中至少两种抗体（IIC3.7和IIC4.6）可保护小鼠免受同源血清型细菌的攻击感染，但不能保护小鼠免受异源血清型细菌的攻击感染。一种与分离的M蛋白在高稀释度（1:204,800）下发生反应的杂交瘤抗体，未能与24型链球菌表面的M蛋白发生反应，因此不能调理同源菌，也不能保护小鼠免受攻击感染。由于杂交瘤抗体针对单个独特的免疫决定簇，这些结果表明，保护性免疫可能针对暴露在强毒A组链球菌表面的M蛋白的几种不同抗原决定簇中的任何一种。
