氯胺-T处理的组织样本中氧化钙调蛋白的放射免疫分析

Radioimmunoassay of oxidized calmodulin in chloramine-T treated tissue samples.

作者信息

Biber A, Hempel K

出版信息

J Clin Chem Clin Biochem. 1984 Feb;22(2):185-8. doi: 10.1515/cclm.1984.22.2.185.

DOI:10.1515/cclm.1984.22.2.185

Abstract

We found that a short incubation of calmodulin with chloramine-T alters its antigenic structure. Antisera elicited by performic acid oxidized calmodulin only bind with chloramine-T treated calmodulin. Based on this finding we have developed an assay for the determination of calmodulin in small tissue samples. Tissue homogenates are incubated with 10 mmol/l chloramine-T before calmodulin quantification. Immune complexes are isolated by sodium sulphate precipitation. [125I]calmodulin is used as tracer.

摘要

我们发现，钙调蛋白与氯胺-T短时间温育会改变其抗原结构。过甲酸氧化的钙调蛋白引发的抗血清仅与经氯胺-T处理的钙调蛋白结合。基于这一发现，我们开发了一种用于测定小组织样本中钙调蛋白的检测方法。在进行钙调蛋白定量之前，将组织匀浆与10 mmol/l氯胺-T温育。通过硫酸钠沉淀分离免疫复合物。使用[125I]钙调蛋白作为示踪剂。