Miller C, White M M
Proc Natl Acad Sci U S A. 1984 May;81(9):2772-5. doi: 10.1073/pnas.81.9.2772.
The inhibition by 4,4'-diisothiocyano-2,2'-stilbenedisulfonate (DIDS) of Cl- channels from Torpedo electroplax incorporated in planar phospholipid bilayer membranes is studied. DIDS irreversibly and rapidly inhibits the macroscopic conductance of membranes containing many channels. At the single-channel level, the effect of DIDS is more complicated. The uninhibited single channel displays three "substates" of conductances 20, 10, and 0 pS. Short exposure (5-30 s) to 10 microM DIDS converts this three-level active channel into a "conventional" channel of 10-pS conductance. Longer exposure eliminates all channel fluctuations. The results are taken as strong evidence that the Cl- channel is constructed as a functional dimer of identical protein subunits.
研究了4,4'-二异硫氰基-2,2'-二苯乙烯二磺酸盐（DIDS）对掺入平面磷脂双层膜中的电鳐电板氯离子通道的抑制作用。DIDS不可逆且迅速地抑制含有许多通道的膜的宏观电导。在单通道水平上，DIDS的作用更为复杂。未受抑制的单通道表现出20、10和0 pS三种“亚状态”的电导。短时间（5 - 30秒）暴露于10 microM DIDS会将这种三电平活性通道转变为电导为10 pS的“常规”通道。长时间暴露则消除所有通道波动。这些结果被视为氯离子通道由相同蛋白质亚基组成功能性二聚体的有力证据。
