Zimmermann W, Scherneck S, Geissler E
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1983 Apr;254(2):187-96.
An enzyme-linked immunosorbent assay (ELISA) for detection of specific IgG antibodies against the capsid antigen of SV40 and SV40-GBM is described. We have determined the presence of SV40 and SV40-GBM IgG antibodies, respectively, in sera of labworkers who are in intensive or rare contact to SV40, in sera of tumor patients as well as in sera of different control persons. In 13% of the sera of the control group antibodies against SV40 were found, when the persons were born 1962 or later. They received SV40-free poliomyelitis vaccine. On the other hand 24% of the sera were positive when the people were born between 1959-61 and probably received SV40-contaminated poliomyelitis vaccine. A similar percentage of positive sera (21-32%) were found in cancer patients suffering on different tumors. Also 29% of the sera of labworkers, born before 1955 and with rare contact to SV40 reacted positive. However, 55% of the sera of labworkers had positive sera when they were in intensive contact to SV40. The results presented and discussed in the light of SV40 as a possible risk factor for the human population.
本文描述了一种用于检测针对SV40和SV40-胶质母细胞瘤（GBM）衣壳抗原的特异性IgG抗体的酶联免疫吸附测定（ELISA）。我们分别在与SV40有密切或极少接触的实验室工作人员血清、肿瘤患者血清以及不同对照组人员血清中检测了SV40和SV40-GBM IgG抗体的存在情况。在对照组中，1962年及以后出生的人，其血清中13%检测到抗SV40抗体，他们接种的是不含SV40的脊髓灰质炎疫苗。另一方面，1959 - 1961年出生且可能接种了受SV40污染的脊髓灰质炎疫苗的人，其血清中有24%呈阳性。在患有不同肿瘤的癌症患者中也发现了类似比例（21 - 32%）的阳性血清。同样，1955年以前出生且与SV40极少接触的实验室工作人员血清中，有29%呈阳性反应。然而，与SV40有密切接触的实验室工作人员血清中，55%呈阳性。本文根据SV40作为人类可能的风险因素这一情况呈现并讨论了这些结果。
