Destrée O H, Bendig M M, De Laaf R T, Koster J G
Biochim Biophys Acta. 1984 Jun 16;782(2):132-41. doi: 10.1016/0167-4781(84)90016-2.
Using previously cloned Xenopus nucleosomal core histone genes as hybridization probes, a genomic DNA library of Xenopus laevis was screened for histone gene clusters. From over 200 histone-gene containing clones identified, 36 were selected as possibly containing H1 histone genes by hybridization to a probe derived from a sea urchin H1 histone gene. These 36 clones were further analyzed by hybrid-selected translation for the definitive presence of H1 histone genes. The genes for three different H1 histone variants were found: H1A , H1B and H1C . Mapping of the histone genes within each clone showed that at least three different gene arrangements can occur within a cluster and that the type of H1 histone variant present in a cluster may be related to the cluster type. S1-mapping experiments indicated that histone genes found in different cluster-types can be expressed in oocytes. Also, the H1 gene found in one cluster-type was expressed in at least three different cell-types: oocytes, gastrula-stage embryos, and erythroblasts.
利用先前克隆的非洲爪蟾核小体核心组蛋白基因作为杂交探针，对非洲爪蟾的基因组DNA文库进行筛选，以寻找组蛋白基因簇。从鉴定出的200多个含组蛋白基因的克隆中，通过与来源于海胆H1组蛋白基因的探针杂交，挑选出36个可能含有H1组蛋白基因的克隆。通过杂交选择翻译对这36个克隆进行进一步分析，以确定H1组蛋白基因的存在。发现了三种不同的H1组蛋白变体基因：H1A、H1B和H1C。对每个克隆中的组蛋白基因进行定位显示，一个基因簇内至少可以出现三种不同的基因排列方式，并且一个基因簇中存在的H1组蛋白变体类型可能与基因簇类型有关。S1定位实验表明，在不同基因簇类型中发现的组蛋白基因可以在卵母细胞中表达。此外，在一种基因簇类型中发现的H1基因至少在三种不同的细胞类型中表达：卵母细胞、原肠胚期胚胎和成红细胞。
