• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

非洲爪蟾组蛋白基因在注射的蛙卵母细胞核中转录所需的启动子序列。

Promoter sequences required for transcription of Xenopus laevis histone genes in injected frog oocyte nuclei.

作者信息

Heindl L M, Weil T S, Perry M

机构信息

Department of Biochemistry and Molecular Biology, University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston 77030.

出版信息

Mol Cell Biol. 1988 Sep;8(9):3676-82. doi: 10.1128/mcb.8.9.3676-3682.1988.

DOI:10.1128/mcb.8.9.3676-3682.1988
PMID:3221862
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365423/
Abstract

Amphibian oogenesis is accompanied by the accumulation of histone mRNA and proteins in the absence of ongoing DNA replication. To begin an analysis of the mechanisms by which histone gene expression is regulated during frog oogenesis and embryogenesis, we used oocyte injection to examine the upstream sequences required for transcription of genes encoding each of the five histone classes. We found that sequences necessary for maximal levels of transcription are located 100 to 200 base pairs upstream of the corresponding start sites. In this region, each promoter examined contains conserved sequence elements, several of which seem to be histone gene class specific, in addition to other, more common sequence elements believed to be used by general transcription factors.

摘要

两栖动物卵子发生过程中，在没有进行性DNA复制的情况下伴随着组蛋白mRNA和蛋白质的积累。为了开始分析在青蛙卵子发生和胚胎发生过程中组蛋白基因表达调控的机制，我们使用卵母细胞注射来检测编码五种组蛋白类别的每个基因转录所需的上游序列。我们发现，最大转录水平所需的序列位于相应起始位点上游100至200个碱基对处。在该区域，每个检测的启动子都包含保守序列元件，除了其他被认为是一般转录因子使用的更常见序列元件外，其中几个似乎是组蛋白基因类别特异性的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32cb/365423/45b55f0f345e/molcellb00069-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32cb/365423/632cf347cc94/molcellb00069-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32cb/365423/56e19f279aa2/molcellb00069-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32cb/365423/45b55f0f345e/molcellb00069-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32cb/365423/632cf347cc94/molcellb00069-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32cb/365423/56e19f279aa2/molcellb00069-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/32cb/365423/45b55f0f345e/molcellb00069-0119-a.jpg

相似文献

1
Promoter sequences required for transcription of Xenopus laevis histone genes in injected frog oocyte nuclei.非洲爪蟾组蛋白基因在注射的蛙卵母细胞核中转录所需的启动子序列。
Mol Cell Biol. 1988 Sep;8(9):3676-82. doi: 10.1128/mcb.8.9.3676-3682.1988.
2
A variant octamer motif in a Xenopus H2B histone gene promoter is not required for transcription in frog oocytes.非洲爪蟾H2B组蛋白基因启动子中的一种变异八聚体基序对于爪蟾卵母细胞中的转录并非必需。
Mol Cell Biol. 1991 Feb;11(2):641-54. doi: 10.1128/mcb.11.2.641-654.1991.
3
A role for transcription and FRGY2 in masking maternal mRNA within Xenopus oocytes.转录和FRGY2在非洲爪蟾卵母细胞中掩盖母源mRNA的作用。
Cell. 1994 Jun 17;77(6):931-41. doi: 10.1016/0092-8674(94)90141-4.
4
Transcription of a cloned Xenopus laevis H4 histone gene in the homologous frog oocyte system depends on an evolutionary conserved sequence motif in the -50 region.克隆的非洲爪蟾H4组蛋白基因在同源蛙卵母细胞系统中的转录依赖于-50区域的一个进化保守序列基序。
Nucleic Acids Res. 1983 Dec 20;11(24):8641-57. doi: 10.1093/nar/11.24.8641.
5
Histone H2B gene transcription during Xenopus early development requires functional cooperation between proteins bound to the CCAAT and octamer motifs.非洲爪蟾早期发育过程中组蛋白H2B基因转录需要与CCAAT和八聚体基序结合的蛋白质之间的功能协作。
Mol Cell Biol. 1992 Oct;12(10):4400-11. doi: 10.1128/mcb.12.10.4400-4411.1992.
6
Xenopus laevis B4, an intron-containing oocyte-specific linker histone-encoding gene.非洲爪蟾B4，一个含内含子的卵母细胞特异性连接组蛋白编码基因。
Gene. 1994 Jun 10;143(2):233-8. doi: 10.1016/0378-1119(94)90102-3.
7
Regulation of the Xenopus laevis transcription factor IIIA gene during oogenesis and early embryogenesis: negative elements repress the O-TFIIIA promoter in embryonic cells.非洲爪蟾卵母细胞发生和早期胚胎发生过程中III型转录因子A基因的调控：负调控元件抑制胚胎细胞中的O-TFIIIA启动子。
Dev Biol. 1991 Jun;145(2):241-54. doi: 10.1016/0012-1606(91)90123-k.
8
Characterization of somatic cell nuclear reprogramming by oocytes in which a linker histone is required for pluripotency gene reactivation.卵母细胞中连接组蛋白对于多能性基因重新激活的体细胞细胞核重编程的特征。
Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5483-8. doi: 10.1073/pnas.1000599107. Epub 2010 Mar 8.
9
Read-through histone transcripts containing 3' adenylate tails are zygotically expressed in Xenopus embryos and undergo processing to mature transcripts when introduced into oocyte nuclei.
Biochem Biophys Res Commun. 2003 May 16;304(4):612-8. doi: 10.1016/s0006-291x(03)00639-9.
10
Stimulation of sea urchin H2B histone gene transcription by a chromatin-associated protein fraction depends on gene sequences downstream of the transcription start site.一种与染色质相关的蛋白质组分对海胆H2B组蛋白基因转录的刺激作用取决于转录起始位点下游的基因序列。
Mol Cell Biol. 1985 Oct;5(10):2764-9. doi: 10.1128/mcb.5.10.2764-2769.1985.

引用本文的文献

1
Molecular characterization and cytogenetic analysis of highly repeated DNAs of lake trout, Salvelinus namaycush.湖红点鲑（Salvelinus namaycush）高度重复DNA的分子特征及细胞遗传学分析
Chromosoma. 1995 Dec;104(4):242-51. doi: 10.1007/BF00352255.
2
Interaction of the CCAAT displacement protein with shared regulatory elements required for transcription of paired histone genes.CCAAT 位移蛋白与成对组蛋白基因转录所需的共享调控元件之间的相互作用。
Mol Cell Biol. 1995 Jul;15(7):3587-96. doi: 10.1128/MCB.15.7.3587.
3
A variant octamer motif in a Xenopus H2B histone gene promoter is not required for transcription in frog oocytes.

本文引用的文献

1
Histone synthesis during the development of Xenopus.非洲爪蟾发育过程中的组蛋白合成。
FEBS Lett. 1980 Nov 17;121(1):1-10. doi: 10.1016/0014-5793(80)81252-x.
2
Transcriptional fidelity of histone genes injected into Xenopus oocyte nuclei.注射到非洲爪蟾卵母细胞核中的组蛋白基因的转录保真度。
Nature. 1980 Nov 6;288(5786):100-2. doi: 10.1038/288100a0.
3
Use of a cloned library for the study of abundant poly(A)+RNA during Xenopus laevis development.利用克隆文库研究非洲爪蟾发育过程中丰富的多聚腺苷酸（poly(A)+）RNA 。
非洲爪蟾H2B组蛋白基因启动子中的一种变异八聚体基序对于爪蟾卵母细胞中的转录并非必需。
Mol Cell Biol. 1991 Feb;11(2):641-54. doi: 10.1128/mcb.11.2.641-654.1991.
4
Histone H2B gene transcription during Xenopus early development requires functional cooperation between proteins bound to the CCAAT and octamer motifs.非洲爪蟾早期发育过程中组蛋白H2B基因转录需要与CCAAT和八聚体基序结合的蛋白质之间的功能协作。
Mol Cell Biol. 1992 Oct;12(10):4400-11. doi: 10.1128/mcb.12.10.4400-4411.1992.
Dev Biol. 1980 May;76(2):449-64. doi: 10.1016/0012-1606(80)90393-0.
4
Delimitation of far upstream sequences required for maximal in vitro transcription of an H2A histone gene.H2A组蛋白基因体外最大转录所需的远上游序列的界定
Proc Natl Acad Sci U S A. 1982 Jan;79(2):297-301. doi: 10.1073/pnas.79.2.297.
5
Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.T-A-T-A-A-A-T-A序列上游的间隔DNA序列对于体内H2A组蛋白基因转录的促进至关重要。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7102-6. doi: 10.1073/pnas.77.12.7102.
6
The organization and expression of histone gene families.组蛋白基因家族的组织与表达
Cell. 1981 Aug;25(2):301-13. doi: 10.1016/0092-8674(81)90048-9.
7
Histone gene expression in early development of Xenopus laevis. Analysis of histone mRNA in oocytes and embryos by blot-hybridization and cell-free translation.非洲爪蟾早期发育过程中的组蛋白基因表达。通过印迹杂交和无细胞翻译分析卵母细胞和胚胎中的组蛋白mRNA。
Differentiation. 1983;24(3):226-33. doi: 10.1111/j.1432-0436.1983.tb01324.x.
8
Expression and organization of histone genes.组蛋白基因的表达与组织
Annu Rev Genet. 1983;17:239-77. doi: 10.1146/annurev.ge.17.120183.001323.
9
Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements.免疫球蛋白κ基因的正确转录需要一个包含保守序列元件的上游片段。
Nature. 1984;310(5972):71-4. doi: 10.1038/310071a0.
10
Organization of Xenopus histone gene variants within clusters and their transcriptional expression.非洲爪蟾组蛋白基因变体在簇内的组织及其转录表达。
Biochim Biophys Acta. 1984 Jun 16;782(2):132-41. doi: 10.1016/0167-4781(84)90016-2.