Shillitoe E J, Greenspan D, Greenspan J S, Silverman S
Cancer. 1984 Jul 15;54(2):266-73. doi: 10.1002/1097-0142(19840715)54:2<266::aid-cncr2820540214>3.0.co;2-#.
HEp-2 cells were infected with herpes simplex virus type 1 (HSV-1) and harvested at selected times thereafter. IgG, IgA, and IgM antibody to virus antigens present in these cells at each time was measured in sera from oral cancer patients, and in matched controls. The IgA response of oral cancer patients was significantly greater than that of controls both at 8 and at 48 hours after infection, but showed no difference in response to HSV-1 virus particles. IgM antibody detected two peaks of antigen synthesis, at 4 and 48 hours after infection. Oral cancer patients had a stronger IgM response than did controls to both early and late peaks; the latter was significant at the 5% level. Oral cancer patients also had a significantly higher IgM response to the virus particle. These results imply the existence of at least two different HSV-1 antigens associated with oral cancer. Both are late antigens; one is recognized by IgA, and the other is recognized by IgM antibody.
将人喉表皮癌细胞（HEp - 2细胞）用1型单纯疱疹病毒（HSV - 1）感染，之后在选定的时间进行收获。在这些细胞中，每次针对病毒抗原的IgG、IgA和IgM抗体，分别在口腔癌患者血清以及配对对照血清中进行测定。口腔癌患者的IgA反应在感染后8小时和48小时均显著强于对照组，但在对HSV - 1病毒颗粒的反应上未显示出差异。IgM抗体检测到感染后4小时和48小时出现两个抗原合成高峰。口腔癌患者对早期和晚期高峰的IgM反应均强于对照组；后者在5%水平具有显著性。口腔癌患者对病毒颗粒的IgM反应也显著更高。这些结果表明，至少存在两种与口腔癌相关的不同HSV - 1抗原。二者均为晚期抗原；一种被IgA识别，另一种被IgM抗体识别。
