Schulz R, Schlaghecke R
Gen Comp Endocrinol. 1984 Apr;54(1):132-8. doi: 10.1016/0016-6480(84)90208-9.
A gonadotropin-receptor preparation from trout during full spermatogenesis known to bind 125I-hCG was used to test the ability of trout pituitary proteins to dislocate hCG from the receptors. From the proteins of male trout pituitaries chromatographed on immobilized concanavalin A, only the glycoprotein fraction was able to compete with hCG for the receptors. Unabsorbed proteins eluting in three fractions were radioiodinated and used as ligands. The fact that they were not taken up specifically by the receptor preparation, and the competition of the glycoproteins with 125I-hCG, is discussed in the context of the vertebrate's gonadotropic hormone system.
一种来自处于完全精子发生期的鳟鱼的促性腺激素受体制剂，已知其能结合125I-hCG，被用于测试鳟鱼垂体蛋白将hCG从受体上置换下来的能力。在固定化伴刀豆球蛋白A上进行层析的雄性鳟鱼垂体蛋白中，只有糖蛋白部分能够与hCG竞争受体。以三个组分洗脱的未吸附蛋白经放射性碘化后用作配体。结合脊椎动物促性腺激素系统的背景，讨论了这些蛋白未被受体制剂特异性摄取以及糖蛋白与125I-hCG竞争的情况。
