Schlaghecke R
Gen Comp Endocrinol. 1983 Feb;49(2):261-9. doi: 10.1016/0016-6480(83)90143-0.
Homogenates of maturing rainbow trout testes show specific binding sites for 125J-labeled hCG (= 125I-labeled hCG). The binding is competitively inhibited by unlabeled hCG and by a hypophyseal extract of rainbow trout. It could be demonstrated that the tissue 125J-hCG binding specificity is restricted to the gonadal preparation. The trout testis was characterized by determining affinity and capacity from Scatchard plot analysis giving a high constant of dissociation Kd 3.65 x 10(-10)/M and a low binding capacity of 0.88 x 10(-15) M/mg tissue. The test system is markedly dependent on temperature, incubation-time, and pH. The maximum binding was found at 37 degrees during 2 hr of incubation in a buffer of pH 7.5.
成熟虹鳟鱼睾丸的匀浆显示出对125I标记的人绒毛膜促性腺激素（= 125I标记的hCG）的特异性结合位点。未标记的hCG和虹鳟鱼的垂体提取物可竞争性抑制这种结合。可以证明，组织125I-hCG结合特异性仅限于性腺制剂。通过Scatchard图分析确定亲和力和容量，对虹鳟鱼睾丸进行表征，得到高解离常数Kd 3.65×10（-10）/M和低结合容量0.88×10（-15）M/mg组织。该测试系统明显依赖于温度、孵育时间和pH值。在pH 7.5的缓冲液中孵育2小时期间，在37摄氏度时发现最大结合。
