蓝藻（蓝细菌）中依赖氧气的质子外流
Oxygen-dependent proton efflux in cyanobacteria (blue-green algae).
作者信息
Scherer S, Stürzl E, Böger P
出版信息
J Bacteriol. 1984 May;158(2):609-14. doi: 10.1128/jb.158.2.609-614.1984.
Abstract
The oxygen-dependent proton efflux (in the dark) of intact cells of Anabaena variabilis and four other cyanobacteria (blue-green algae) was investigated. In contrast to bacteria and isolated mitochondria, an H+/e ratio (= protons translocated per electron transported) of only 0.23 to 0.35 and a P/e ratio of 0.8 to 1.5 were observed, indicative of respiratory electron transport being localized essentially on the thylakoids, not on the cytoplasmic membrane. Oxygen-induced acidification of the medium was sensitive to cyanide and the uncoupler carbonyl cyanide m-chlorophenylhydrazone. Inhibitors such as 2,6-dinitrophenol and vanadate exhibited a significant decrease in the H+/e ratio. After the oxygen pulse, electron transport started immediately, but proton efflux lagged 40 to 60 s behind, a period also needed before maximum ATP pool levels were attained. We suggest that proton efflux in A. variabilis is due to a proton-translocating ATP hydrolase (ATP-consuming ATPase) rather than to respiratory electron transport located on the cytoplasmic membrane.
摘要
研究了可变鱼腥藻及其他四种蓝细菌（蓝藻）完整细胞（在黑暗中）的氧依赖质子外流。与细菌和分离的线粒体不同，观察到的H⁺/e比值（=每传递一个电子转运的质子）仅为0.23至0.35，P/e比值为0.8至1.5，这表明呼吸电子传递基本上定位于类囊体上，而非细胞质膜上。氧诱导的培养基酸化对氰化物和解偶联剂间氯苯腙敏感。诸如2,6 -二硝基苯酚和钒酸盐等抑制剂使H⁺/e比值显著降低。氧脉冲后，电子传递立即开始，但质子外流滞后40至60秒，达到最大ATP库水平也需要这一时间段。我们认为可变鱼腥藻中的质子外流是由于质子转运ATP水解酶（消耗ATP的ATP酶），而非位于细胞质膜上的呼吸电子传递。
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