Lin Q, Blaisdell J, O'Keefe E, Earp H S
J Cell Physiol. 1984 Jun;119(3):267-72. doi: 10.1002/jcp.1041190304.
Hydrocortisone and dexamethasone produced a time-dependent increase [125I]epidermal growth factor [( 125I]EGF) binding in primary cultures of isolated rat hepatocytes. Maximally effective doses of glucocorticoids resulted in a 70-100% increase in binding. The effect was similar when hepatocytes were maintained on collagen-coated plates or directly on culture dishes. The glucocorticoid-mediated increase in [125I]EGF binding could be detected after 4 h exposure to glucocorticoid and was substantial by 8 h. The major effect of glucocorticoid appeared to be to increase the number of EGF receptors. While insulin (100 nM) had no effect on basal [125I]EGF binding, it significantly inhibited the increase produced by the glucocorticoid. Since the inhibitory effect of insulin was only observed when insulin was added with the inducing glucocorticoid, insulin appears to inhibit an early hydrocortisone-mediated event.
氢化可的松和地塞米松使原代培养的分离大鼠肝细胞中[125I]表皮生长因子（[125I]EGF）结合呈时间依赖性增加。糖皮质激素的最大有效剂量导致结合增加70 - 100%。当肝细胞培养于胶原包被平板上或直接培养于培养皿中时，效果相似。糖皮质激素介导的[125I]EGF结合增加在接触糖皮质激素4小时后即可检测到，8小时时显著增加。糖皮质激素的主要作用似乎是增加EGF受体的数量。虽然胰岛素（100 nM）对基础[125I]EGF结合无影响，但它显著抑制了糖皮质激素产生的增加。由于胰岛素的抑制作用仅在与诱导性糖皮质激素同时添加时才观察到，则胰岛素似乎抑制了氢化可的松介导的早期事件。
