Ando Y, Tsuzuki T
J Gen Microbiol. 1984 Feb;130(2):267-73. doi: 10.1099/00221287-130-2-267.
The surface charge against pH behaviour of spores of Clostridium perfringens type A was determined using a colloid titration method in the pH range 4-9. The native spores as well as various ionic forms of the spores loaded with divalent cations were found to be negatively charged. A positive colloid, MGCh, inhibited the ionic germination of the spores and this inhibition was highly dependent on pH. However, another positive colloid, GCh, did not inhibit the germination. Chemical modification of carboxyl groups on the spore surface by use of a water-soluble carbodiimide with two nucleophiles, glycine ethyl ester and taurine, caused spores not only to have little or no colloidal charge but also to lose the ability to germinate. The modification of carboxyl groups in the spore coat protein also resulted in elimination of, or marked reduction in its negative charge. These results suggest that carboxyl groups in the spore coat protein are the major negatively charged species on the spore surface. The role of surface charge in the ionic germination of the spores is discussed.
采用胶体滴定法在4-9的pH范围内测定了A型产气荚膜梭菌孢子的表面电荷随pH的变化行为。发现天然孢子以及负载二价阳离子的各种离子形式的孢子均带负电荷。一种正胶体MGCh抑制了孢子的离子萌发，且这种抑制高度依赖于pH。然而，另一种正胶体GCh则不抑制萌发。通过使用带有两种亲核试剂（甘氨酸乙酯和牛磺酸）的水溶性碳二亚胺对孢子表面的羧基进行化学修饰，不仅使孢子几乎没有或没有胶体电荷，还使其失去了萌发能力。孢子 coat 蛋白中羧基的修饰也导致其负电荷消除或显著减少。这些结果表明，孢子 coat 蛋白中的羧基是孢子表面主要的带负电物质。讨论了表面电荷在孢子离子萌发中的作用。
