Sacks L E
Biochim Biophys Acta. 1981 Apr 17;674(1):118-27. doi: 10.1016/0304-4165(81)90353-6.
Bacterial endospore germination is powerfully influenced by inorganic salts, cations having especially important effects. Spores of Clostridium perfringens 8-6 are unusual in lacking a spore coat; these spores germinate only in the presence of lysozyme, which readily digests the exposed cortex. Lysozyme-induced germination showed the same response to ionic strength and valence of cations as does lysozyme hydrolysis of peptidoglycan, and close parallels are evident in the influence of inorganic cations on germination of normal spores. La3+ and transition element cations inhibited lysozyme-induced germination at low concentration, again demonstrating parallels with their action on lysozyme digestion of peptidoglycan and on the germination of normal spores. The poly-cations poly(L-lysine) and Ruthenium Red inhibited at extremely low concentrations. Mn2+ and Co2+, at appropriately low concentrations, stimulated lysozyme germination of 8-6 spores and also lysis of Micrococcus lysodeikticus.
细菌芽孢萌发受到无机盐的强烈影响，阳离子的影响尤为重要。产气荚膜梭菌8 - 6的芽孢不同寻常之处在于缺乏芽孢衣；这些芽孢仅在溶菌酶存在的情况下才会萌发，溶菌酶能轻易消化暴露的皮层。溶菌酶诱导的萌发对离子强度和阳离子价态的反应与溶菌酶对肽聚糖的水解反应相同，并且在无机阳离子对正常芽孢萌发的影响方面也有明显的相似之处。La3 +和过渡元素阳离子在低浓度时抑制溶菌酶诱导的萌发，这再次证明了它们在对肽聚糖的溶菌酶消化作用以及对正常芽孢萌发作用方面的相似性。聚阳离子聚（L - 赖氨酸）和钌红在极低浓度下具有抑制作用。Mn2 +和Co2 +在适当的低浓度下，刺激8 - 6芽孢的溶菌酶萌发，也刺激溶壁微球菌的裂解。
