自发性高血压大鼠脑微血管中的血管紧张素转换酶活性降低。

Angiotensin-converting enzyme activity is reduced in brain microvessels of spontaneously hypertensive rats.

作者信息

Kobayashi H, Take K, Wada A, Izumi F, Magnoni M S

出版信息

J Neurochem. 1984 Jun;42(6):1655-8. doi: 10.1111/j.1471-4159.1984.tb12756.x.

DOI:10.1111/j.1471-4159.1984.tb12756.x

Abstract

Angiotensin -converting enzyme (ACE) activity in brain microvessels of spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) controls was measured. Cerebral microvessels, prepared from the cerebral cortices by the albumin flotation and glass bead filtration technique, were free of neuronal and glial elements. ACE activity in brain microvessels of SHR was lower than that of WKY. A Woolf - Augustinsson -Hofstee plot showed that the reduction of the enzyme activity in SHR was due to a 30% decrease in Vmax, without any change in Km for substrate. The decrease of ACE activity in brain microvessels of SHR may indicate an impairment of the central renin-angiotensin system and may be related to cerebral microvascular dysfunctions occurring in hypertension.

摘要

测定了自发性高血压大鼠（SHR）和Wistar Kyoto（WKY）对照大鼠脑微血管中的血管紧张素转换酶（ACE）活性。采用白蛋白漂浮和玻璃珠过滤技术从大脑皮层制备的脑微血管不含神经元和神经胶质成分。SHR脑微血管中的ACE活性低于WKY。Woolf-Augustinsson-Hofstee图显示，SHR中酶活性的降低是由于Vmax降低了30%，而底物的Km没有任何变化。SHR脑微血管中ACE活性的降低可能表明中枢肾素-血管紧张素系统受损，并且可能与高血压中发生的脑微血管功能障碍有关。