Wakayama Y, Okayasu H, Kumagai T
J Neurol Sci. 1984 Mar;63(3):411-21. doi: 10.1016/0022-510x(84)90164-3.
The numerical change of intramembranous particles (IMP) and square arrays (SA) was investigated in the muscle plasma membrane after anoxia. The proximal portion of the hind limb of Wistar rats weighing 250-350 g was strongly tied with steel wire. The contralateral hind limb was served as control. After 3 and 6 h, the extensor digitorum longus (EDL) muscles of both control and anoxic groups were studied by freeze-fracture electron microscopy. The findings of anoxic EDL plasma membrane included the presence of band-like or patchy form particle free areas with the tendency to increase from 3 to 6 h and the preservation of SA even after 6 h of anoxia. However, the IMP and caveolae densities between control and anoxic groups were not statistically different. The group median densities of SA/micron2 of control P face were 4.9 with midrange of 2.2-9.7 and 7.1 with mid-range of 2.7-10.9 after 3 and 6 h; whereas those of anoxic P face were 6.0 with mid-range of 1.8-8.2 (P greater than 0.1) and 6.9 with mid-range of 3.3-13.5 (P greater than 0.1), respectively. These changes are quite different from those of muscle plasma membrane in Duchenne muscular dystrophy.
研究了缺氧后肌细胞膜内膜颗粒（IMP）和方形阵列（SA）的数量变化。用钢丝紧紧捆绑体重250 - 350克的Wistar大鼠后肢近端部分。对侧后肢作为对照。3小时和6小时后，通过冷冻断裂电子显微镜研究对照和缺氧组的趾长伸肌（EDL）。缺氧EDL质膜的研究结果包括出现带状或片状无颗粒区，且从3小时到6小时有增加趋势，即使缺氧6小时后SA仍保留。然而，对照和缺氧组之间IMP和小窝的密度没有统计学差异。对照P面3小时和6小时后SA/μm²的组中位数密度分别为4.9（范围2.2 - 9.7）和7.1（范围2.7 - 10.9）；而缺氧P面分别为6.0（范围1.8 - 8.2，P大于0.1）和6.9（范围3.3 - 13.5，P大于0.1）。这些变化与杜氏肌营养不良症中肌细胞膜的变化有很大不同。
