Olson D M, Lye S J, Skinner K, Challis J R
J Reprod Fertil. 1984 May;71(1):45-55. doi: 10.1530/jrf.0.0710045.
The changes with time in intrauterine tissue production and concentrations of PGE-2, PGF-2 alpha, 6-keto PGF-1 alpha and PGFM (13,14-dihydro-15-keto PGF-2 alpha) in maternal and fetal plasma and amniotic fluid were investigated during the first 72 h of pulsatile administration of ACTH1-24 to chronically catheterized fetal sheep. By 72 h there were no changes in the frequency, maximum amplitude or duration of uterine contractions compared to preinfusion values. Basal concentrations of PGE-2 in maternal and fetal plasma were generally higher than those of PGF-2 alpha, while 6-keto PGF-1 alpha values were intermediate. The concentrations of all PGs increased in amniotic fluid during ACTH infusion. In fetal plasma and in maternal vena caval plasma, however, there were significant increases only in PGF-2 alpha and PGFM. No changes were observed in plasma concentrations for any PG during saline infusion. The mean output of PGE-2, PGF-2 alpha and 6-keto PGF-1 alpha by dispersed cells prepared from chorioallantois and fetal and maternal cotyledons was consistently higher after ACTH for 72 h than from saline-infused animals, although significance (P less than 0.05) was achieved only for PGE-2 in chorioallantois . There are 3 conclusions. (1) Increases in ovine intrauterine tissue PG production precede the occurrence of increased myometrial contractile activity after ACTH treatment of fetal sheep. The results imply a causal relationship between rising PG and later myometrial contractions, rather than PG changes resulting from enhanced uterine activity. (2) The major site(s) of increased PG output in vitro from endogenous precursors are fetal structures, especially the chorio-allantoic membranes. (3) Although PGE-2 may be the major circulating PG during late gestation, there is a selective increase in plasma PGF-2 alpha concentrations before the onset of delivery.
在对长期插管的胎羊进行促肾上腺皮质激素1 - 24脉冲给药的前72小时内，研究了母羊和胎儿血浆及羊水中宫内组织产生的前列腺素E-2（PGE-2）、前列腺素F-2α（PGF-2α）、6-酮前列腺素F-1α（6-keto PGF-1α）和前列腺素FM（13,14-二氢-15-酮前列腺素F-2α，PGFM）随时间的变化。到72小时时，与输注前的值相比，子宫收缩的频率、最大幅度或持续时间没有变化。母羊和胎儿血浆中PGE-2的基础浓度通常高于PGF-2α，而6-酮前列腺素F-1α的值介于两者之间。在促肾上腺皮质激素输注期间，羊水中所有前列腺素的浓度均升高。然而，在胎儿血浆和母羊腔静脉血浆中，仅PGF-2α和PGFM显著增加。在输注生理盐水期间，未观察到任何前列腺素的血浆浓度有变化。在促肾上腺皮质激素作用72小时后，从绒毛膜尿囊、胎儿和母羊子叶制备的分散细胞中，PGE-2、PGF-2α和6-酮前列腺素F-1α的平均产量始终高于输注生理盐水的动物，尽管仅绒毛膜尿囊中PGE-2达到了显著性（P＜0.05）。有三个结论。（1）在对胎羊进行促肾上腺皮质激素治疗后，绵羊宫内组织前列腺素产量的增加先于子宫肌层收缩活性的增加。结果表明前列腺素水平升高与随后的子宫肌层收缩之间存在因果关系，而不是子宫活动增强导致前列腺素变化。（2）体外由内源性前体增加前列腺素产量的主要部位是胎儿结构，尤其是绒毛膜尿囊膜。（3）虽然在妊娠后期PGE-2可能是主要的循环前列腺素，但在分娩开始前，血浆中PGF-2α浓度有选择性增加。
