Armstrong K A, Acosta R, Ledner E, Machida Y, Pancotto M, McCormick M, Ohtsubo H, Ohtsubo E
J Mol Biol. 1984 May 25;175(3):331-48. doi: 10.1016/0022-2836(84)90352-8.
Nucleotide sequences were determined for a region essential for autonomous replication and partitioning of pSC101, a plasmid whose replication is dependent on the Escherichia coli dnaA gene product. The essential replication region contains one long coding sequence, rep101 , for a protein composed of 316 amino acids, and a polypeptide approximately 37 X 10(3) Mr in size was identified as the rep101 gene product. rep101 is preceded by two inverted repeat sequences, three directly repeated sequences and a region of high A + T content containing a sequence similar to the E. coli oriC consensus sequence. Because the lesions in seven replication-deficient insertion mutants, four mutants with increased copy number and one temperature-sensitive replication mutant occur within rep101 , the rep101 gene product must control pSC101 replication and copy number. par, a region adjacent to the replication region, which functions in stable plasmid inheritance, contains several inverted repeat sequences.
测定了pSC101自主复制和分配所必需区域的核苷酸序列，pSC101是一种复制依赖于大肠杆菌dnaA基因产物的质粒。必需的复制区域包含一个长编码序列rep101，其编码一种由316个氨基酸组成的蛋白质，并且一种大小约为37×10³Mr的多肽被鉴定为rep101基因产物。rep101之前有两个反向重复序列、三个直接重复序列以及一个高A+T含量区域，该区域包含一个与大肠杆菌oriC共有序列相似的序列。由于七个复制缺陷插入突变体、四个拷贝数增加的突变体和一个温度敏感复制突变体中的损伤发生在rep101内，所以rep101基因产物必定控制pSC101的复制和拷贝数。par是与复制区域相邻的一个区域，在稳定的质粒遗传中起作用，它包含几个反向重复序列。
