Adlercreutz H, Vähäpassi J
J Steroid Biochem. 1984 Apr;20(4B):963-70. doi: 10.1016/0022-4731(84)90005-0.
In order to study the biological effects of estriol in women 20 mg estriol was administered daily to 7 young women. Plasma luteinizing hormone (LH), estradiol (E2), progesterone (Pg) and prolactin (Prl) were measured during a treatment and a control cycle every second or third day. Further 3, 6 or 20 mg estriol was administered in a single dose to 5 women and plasma Prl, unconjugated and conjugated estriol (E3) measured over 24 h at 2-3 intervals. In 2 experiments with 20 mg E3, blood samples were taken more frequently, over 6 h. When 20 mg E2 was administered daily, 2 of the 7 young women had anovulatory cycles. The mean plasma E2 was lower during the follicular and ovulatory phases (P less than 0.025) and mean plasma LH was higher (P less than 0.005) during the luteal phase, when E3 was given. Because of the 2 anovulatory cycles the mean Pg value during the luteal phase was lower (P less than 0.05) during treatment. There was a slight decrease in mean Prl in 5 out of 6 women (P less than 0.0005), but in only 1 woman was this decrease substantial (from a mean value of 27.6-18.9 ng/ml; P less than 0.01). When 6 or 20 mg E3 was administered orally in the morning a significant negative correlation (P less than 0.01) between plasma Prl and unconjugated E3 was found. The correlation coefficient was highest (r = -0.74) with 6 mg E3. When 3 mg was administered no obvious effect on Prl section was seen. However, when results from all experiments with identical time schedules were pooled (two with 3 mg, two with 6 mg and one with 20 mg E3) and the mean values for plasma Prl calculated and compared with the mean values obtained in 7 control experiments, it was found that E3 administration in the morning almost abolishes the Prl rise during the following night. There was a statistically significant (P less than 0.0125) decrease in the difference between the maximum value during the night and the minimum value during the day. The minimum value was significantly higher (P less than 0.01) and the maximum value significantly lower (P less than 0.025) after E3 treatment, compared to the control values. It is concluded that long-term administration of 20 mg E3 usually has only a slight but significant decreasing effect on mean plasma Prl concentration measured in the morning, before the next dose is taken.(ABSTRACT TRUNCATED AT 400 WORDS)
为研究雌三醇对女性的生物学效应，对7名年轻女性每日给予20mg雌三醇。在治疗周期和对照周期中，每隔第二天或第三天测定血浆促黄体生成素（LH）、雌二醇（E2）、孕酮（Pg）和催乳素（Prl）。另外，对5名女性单次给予3mg、6mg或20mg雌三醇，并在24小时内每隔2 - 3小时测定血浆Prl、未结合和结合雌三醇（E3）。在两项使用20mg E3的实验中，采血更频繁，持续6小时。每日给予20mg E2时，7名年轻女性中有2人出现无排卵周期。给予E3时，卵泡期和排卵期的平均血浆E2较低（P<0.025），黄体期的平均血浆LH较高（P<0.005）。由于2个无排卵周期，治疗期间黄体期的平均Pg值较低（P<0.05）。6名女性中有5名的平均Prl略有下降（P<0.0005），但只有1名女性的下降幅度较大（从平均值27.6降至18.9ng/ml；P<0.01）。早晨口服6mg或20mg E3时，血浆Prl与未结合E3之间存在显著负相关（P<0.01）。给予6mg E3时相关系数最高（r = -0.74）。给予3mg时，对Prl水平未见明显影响。然而，当汇总所有时间安排相同的实验结果（两项使用3mg，两项使用6mg，一项使用20mg E3），计算血浆Prl的平均值并与7项对照实验获得的平均值进行比较时，发现早晨给予E3几乎消除了随后夜间Prl的升高。夜间最大值与白天最小值之间的差异有统计学意义的降低（P<0.0125）。与对照值相比，E3治疗后最小值显著升高（P<0.01），最大值显著降低（P<0.025）。结论是，长期给予20mg E3通常对下次给药前早晨测定的平均血浆Prl浓度仅有轻微但显著的降低作用。（摘要截短至400字）
