Weatherby R P, Allan R D, Johnston G A
J Neurosci Methods. 1984 Jan;10(1):23-8. doi: 10.1016/0165-0270(84)90076-1.
The GABA analogue baclofen [3-(p-chlorophenyl)-4-aminobutanoic acid] has stereospecific actions on the peripheral and central nervous systems. This paper describes the resolution of tritium-labelled baclofen by high performance liquid chromatography on a reverse-phase C18 column using a chiral mobile phase. The method, which may have general application to certain other GABA analogues, affords optically pure (+)- and (-)-baclofen labelled with tritium to high specific activity suitable for ligand binding and other neurochemical studies.
γ-氨基丁酸类似物巴氯芬[3-(对氯苯基)-4-氨基丁酸]对周围和中枢神经系统具有立体特异性作用。本文描述了使用手性流动相通过反相C18柱上的高效液相色谱法拆分氚标记的巴氯芬。该方法可能普遍适用于某些其他γ-氨基丁酸类似物，可提供具有高比活度的光学纯(+)-和(-)-巴氯芬，其标记有氚，适用于配体结合和其他神经化学研究。
