Wuis E W, Dirks R J, Vree T B, van der Kleyn E
J Chromatogr. 1985 Feb 8;337(2):341-50. doi: 10.1016/0378-4347(85)80047-5.
A reversed-phase high-performance liquid chromatographic method for the determination of the skeletal muscle relaxant baclofen in human plasma and urine is described. Cation-exchange extraction, precolumn derivatization with o-phthaldialdehyde, and on-column concentration precede fluorimetric detection (excitation at 340 nm, emission at 460 nm). The precision of the assay was always better than 6%. Recoveries of standards added to plasma and urine were 92% and 93%, respectively. With a sample size of 0.5 ml, a detection limit of a few nanograms, and the possibility of analysing up to four samples per hour, this method is suitable for pharmacokinetic studies. An example is presented.
本文描述了一种反相高效液相色谱法，用于测定人血浆和尿液中的骨骼肌松弛剂巴氯芬。采用阳离子交换萃取、邻苯二甲醛柱前衍生化和柱上富集，然后进行荧光检测（激发波长340nm，发射波长460nm）。该测定方法的精密度始终优于6%。添加到血浆和尿液中的标准品回收率分别为92%和93%。该方法进样量为0.5ml，检测限为几纳克，每小时最多可分析4个样品，适用于药代动力学研究。文中给出了一个实例。
